慢性乙型肝炎患者外周血分泌白细胞介素-35的B细胞和白细胞介素-35表达水平及其意义  

Expression and role of interleukin 35-producing B cells and interleukin 35 from peripheral blood of patients with chronic hepatitis B

作　　者：刘亚允[1] 余娇 杨阳 蒋蒙 李成忠[1] 梁雪松[1] 

机构地区：[1]长海医院感染科,上海200433 [2]第二军医大学免疫研究所

出　　处：《中华传染病杂志》2017年第9期528-532,共5页Chinese Journal of Infectious Diseases

基　　金：上海市自然科学基金（16ZR1400400,09ZR1400500）;上海市卫生局基金（2012094）

摘　　要：目的明确分泌IL-35的调节性B细胞（i35-Breg）及其效应因子IL-35在CHB患者外周血水平及其与HBVDNA和肝脏炎症程度的相关性。方法选择初治CHB患者35例，普通干扰素治疗的HBeAg阳性CHB患者17例，健康对照者15名，应用流式细胞术和ELISA检测上述研究对象外周血i35-Breg及IL35表达水平。统计学处理采用Kruskal—Wallis检验、Wilcox秩和检验以及两变量相关性分析。结果初治CHB患者外周血i35-Breg为3．05％（0．89％，4．97％），IL-35为2．81μg／L（0．30／μg／L，12．33μg／L），明显高于健康对照者的0．17％（0．13％，0．45％）和0．17μg／L（0，1．93ug／I，），差异有统计学意义（z值分别为-3．309、-2．419，P值分别为0．001、0．016）。初治CHB患者外周血i35Breg细胞水平与HBVDNA呈负相关（r=-0．529，P=0．008），IL-35水平与HBVDNA水平无相关性（r=0．11，P=0．54）。HBeAg阳性初治CHB患者i35Breg水平为3．16％（1．34％，5．62％），IL-35水平为4．58μg／L（0．79μg／L，22．37μg／L），均明显高于健康对照者，差异有统计学意义（F值分别为3．39、3．37，均P〈0．01）。ALT和AsT〈300U／L的CHB患者外周血IL-35水平分别为3．03弘g／L（o．74μg／L，22．37μg／L）和3．25μg／L（0．83μg／L，22．35μg／L），与健康对照者比较差异有统计学意义（F值分别为2．868、3．114，均P〈0．01）。ALT〈300U／L的初治CHB患者外周血i35-Breg细胞水平为3．14％（1．03％，4．65％），与健康对照者比较差异有统计学意义（F=3．219，P=0．004）。干扰素治疗早期患者外周血IL-35水平呈下降趋势，但差异无统计学意义（Х^2=1．45，P=0．48）；持续病毒学应答者基线IL-35水平为0（0，13．33μg／L），低于部分应答或无应答者的0．61μg／L（0，24．72μg／L），但差异无统计学意义（F=0．75，P=0．68）。结论i35-Breg细胞及其效应因子参与慢Objective To explore the level of interleukin 35-producing B cells （i35 Breg） as well as its effect factors, interleukin-35 （IL-35）, in peripheral blood of patients with chronic hepatitis B （CHB）, and their relationship with hepatitis B virus （HBV） DNA and liver inflammatory degree. Methods A total of 35 treatment-naive CHB patients, 17 interferon （IFN）-treated Ht3eAg-positive CHB patients and 15 healthy controls （HC） were enrolled. The levels of i35-Breg and IL-35 in peripheral blood were tested by flow cytometry and enzyme-linked immunosorption assay （ELISA）. Kruskal-Wallis test, Wilcox rank sum test and two variables correlation analysis were used for statistical analysis. Results The percentage of i35 Breg cells as well as IL-35 level in peripheral blood of naive CHB patients were 3.05~ （0. 89~~, 4. 97%） and 2.81 ffg/L （0. 30 μg/L, 12.35 μg/L）, respectively, which were both significantly higher than those in HC group, which were 0.17 % （0.13 %, 0.45%） and 0. 17 fig/L（0, 1.93 fig/L）, respectively. The difference were statistical significant（Z=--3. 309 and --2. 419, respectively, P=0. 001 and 0. 016, respectively）. The peripheral level of i35- Breg was negatively correlated with the viral load in treatment-naive CHB patients （r=--0. 529, P= 0. 008）, while there was no correlation between the peripheral level of IL-35 and the viral load in treatment-naive CHB patients （r=0.11, P= 0.54）. The levels of i35-Breg and IL-35 in HBeAg positive CHB patients were 3.16~//00 （1.34%, 5.62%） and 4.58μg/L （0.79 μg/L, 22.37μg/L）, respectively, which were both higher than those in HC group. The difference was statistically significant （F= 3.39 and 3.37, respectively, both P〈0.01）. Compared to HC group, the IL-35 levels in peripheral blood of CHB patients with ALT and AST levels less than 300 U/L were 3. 03 μg/L （0. 74 μg/L, 22. 37 μg/L） and 3. 25 tzg/L （0.83 μg/L, 22.35 /xg/L）, respectively, with statistically significant difference （F= 2.

关 键 词：肝炎 乙型 慢性 白细胞介素-35 分泌白细胞介素35的B细胞 

分 类 号：R512.62[医药卫生—内科学]