1α,25二羟基维生素D_3对人早幼粒白血病细胞(HL—60)的诱导分化作用  

EFFECT OF 1α, 25-DIHYDROXY-VITAMIN D_3 ON DIFFERENTIATION AND PROLIFERATION OF A HUMAN MYELOID LEUKEMIA CELL LINE

作　　者：薛延[1] 王红霞[2] 袁润英[2] 相东[2] 田洁[3] 李秀森[3] 魏文[3] 

机构地区：[1]北京积水潭医院北京市创伤骨科研究所生化研究室,100035 [2]北京积水潭医院北京市创伤骨科研究所免疫研究室 [3]军事医学科学院基础医学研究所,北京100850

出　　处：《中国药理学通报》1991年第4期267-270,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目

摘　　要：1α,25二羟基维生素D_3在体外可诱导HL-60细胞向单核/巨噬样细胞发展。在形态上,核/质比例减少,核仁减少,细胞变得不规则并伸出伪足;在细胞化学方面,细胞对NBT还原能力增强,α萘酚醋酸酯酶和酸性磷酸酶阳性率明显增加,细胞周期的变化是:二倍体细胞明显增多,G_0+G_1期增加和S期减少。表明在1α,25(OH)_2D_3作用下,HL-60细胞向成熟分化,细胞DNA合成受抑制。1 α 25-dihydroxy-vitamin D3(1α 25 (OH)2 D3)induced differentiation of human promyelocytic leukaemia cells ( HL-60 ) into mature myeloid cells in vitro. The ratio of nuclei to cytoplasma decreased; Their nucleoli reduced; Cells became irrgular and extended the pseudopods. 1 α 25 (OH ) 2 D3 caused significant increase of nitroblue tetrazolium ( NBT ) reduction and α-non-specific acid esterase (α-NAE ) and acid phosphatase (ACP ) activities. These data and morphological characteristics suggest that HL-60 cells were conclusively identified as monocytes/macrophages. The histogram of DNA distribution ahylyzed by flow cytometry demonstrated G0 + G1 phase increased and S phase increased and S phase decreased remarkably after treatment with lα, 25 ( OH ) 2 D3 as compared with untreated cells.

关 键 词：白血病 诱导分化 维生素D3 二羟基 

分 类 号：R979.1[医药卫生—药品]