刚性基底表面图案化氧化石墨烯对细胞粘附行为调控  

Regulation of Cell Adhesion by Surface Patterning Graphene Oxide on Rigid Substrates

作　　者：徐盼举 邢赟 许为中 黄敏 刘爱萍[1,2] 

机构地区：[1]浙江理工大学物理系,杭州310018 [2]浙江理工大学光电材料与器件中心,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2017年第6期778-784,共7页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：国家自然科学基金项目(51272237;51572242);浙江省自然科学基金项目(LY16E020011);浙江理工大学521人才培养计划

摘　　要：生物材料的物理化学性质(如刚度、粗糙度、亲疏水性等)对细胞的粘附、增殖、迁移、分化、凋亡等一系列生命活动会产生重要的影响。此外,对材料进行图案化设计也是调控细胞生命活动的一种有效手段。采用软光刻的方法,以聚二甲基硅氧烷为印章,通过调节压力,获得图案较为规整的氧化石墨烯(GO)阵列。在优化的压力条件下,成功将GO阵列转移至硅、类金刚石膜、氧化铟锡玻璃三种刚性基底表面。利用基底材料与GO材料性能的差异,实现了对神经细胞(PC12)选择性粘附的有效调控。研究结果表明:与刚性基底相比,硬度较小且亲水的GO表面更能有效促进PC12细胞的选择性粘附和增殖。GO阵列表面越大,其表面粘附的细胞密度越大。The physicochemical properties of biological materials（such as stiffness,roughness and hydrophily） have a huge effect on cell behaviors,including adhesion,proliferation, migration,differentiation and apoptosis.The patterning design of biological materials is one of the effective means for regulating cell life activities.In this study,agraphene oxide（GO）array of relatively regular pattern is obtained by pressure regulation with the method of soft lithography with polydimethylsiloxane as stamp.Under the optimized pressure conditions,the GO array is successfully transferred on the surface of three substrates,namely silicon wafer,diamond-like carbon film and indium tin oxide glass.Effective regulation of selective attachment of nerve cell（PC12）has been realized according to the performance difference between substrate material and GO material.The research results show that,comparing with rigid substrate,hydrophilic GO of low hardness performs better in promoting selective adhesion and proliferation of PC12 nerve cells.The bigger the GO array area is,the larger the density of the cells adhered to the surface is.

关 键 词：氧化石墨烯 阵列材料 刚性基底 细胞选择性粘附 

分 类 号：Q689[生物学—生物物理学]