早熟禾种质资源ISSR分子标记的引物筛选  

Primer Screening Study on Bluegrass Germplasm Resources by ISSR molecular markers

作　　者：阿啟兰 刘勇[1] 魏小星[1] 张然 A Qi - lan et al(Qinghai Academy of Animal Science and Veterinary Medicine , Xining, 810016)

机构地区：[1]青藏高原优良牧草种质资源研究省级重点实验室,青海大学畜牧兽医科学院,西宁810016 [2]甘肃农业大学,兰州730070

出　　处：《青海畜牧兽医杂志》2017年第5期1-6,共6页Chinese Qinghai Journal of Animal and Veterinary Sciences

基　　金：青海省青藏高原优良牧草种质资源利用重点实验室(2017-ZJ-Y12);青海省科技厅科技合作专项"青海湖流域沙化土地综合治理技术集成与示范"(2016-HZ-822);"现代农业产业技术体系建设专项资金"(CARS-35-41)

摘　　要：本研究以早熟禾属的60份早熟禾种质资源为材料,参照加拿大哥伦比亚大学提供的100条ISSR引物对其进行PCR扩增,从中筛选出30条多态性丰富、条带清晰稳定、重复性较好的且适合于早熟禾ISSR分析的引物。30条ISSR引物共扩增出135条带,其中多态性条带为56条,多态率为41.5%。本试验筛选出的核心引物可为早熟禾种质资源遗传多样性及育种的研究奠定理论依据。Using the provided by Columbia University 100 ISSR primers for collected from different altitudes of 60 Kentucky bluegrass germplasm resources for PCR amplification,the results show that： there are 30 primers have abundant polymorphism,and amplification of the stripe is clear and bright,can be effectively applied to Kentucky bluegrass ISSR analysis.The total of 30 ISSR primers were expanded to 135 bands,of which the polymorphic stripe was 56,and the polymorphism rate was 41.5%.These core primers will provide a theoretical basis for bluegrass genetic diversity and breeding germplasm resources.

关 键 词：早熟禾种质资源 ISSR 引物筛选 

分 类 号：S543.9[农业科学—作物学]