死亡仔猪脏器中致病性大肠杆菌的分离鉴定及药敏试验  

Isolation and identification of Escherichia coil from case of clinical died swine

作　　者：奎军 简莹娜 张学勇[1] 李秀萍[1] 王戈平[1] 李海基 马利青[1] KUI Jun et al(Qinghai Academy of Animal Science and Veterinary Medicine, Xining, 810016)

机构地区：[1]青海省畜牧兽医科学院,西宁810016 [2]青海省湟中县畜牧兽医站,西宁811600

出　　处：《青海畜牧兽医杂志》2017年第5期23-26,共4页Chinese Qinghai Journal of Animal and Veterinary Sciences

基　　金：<农业基础性长期性科技工作动物疫病观测检测任务>;项目编号:ZX06S2902

摘　　要：从送检的死亡猪组织病料中分离到1株病原菌,经病原菌的分离、生化试验、动物致死试验、药物敏感性试验和PCR扩增及测序,对该菌株进行了鉴定。结果表明:该病原菌为致病性大肠杆菌;药敏试验表明在检测的17种抗生素中该菌株仅对阿奇霉素和克林霉素药物敏感;对强力霉素、多粘菌素B药物中敏;对杆菌肽、氯霉素和多粘附素E等耐药。说明该猪死亡前感染了耐药性较强的致病性大肠杆菌。One pathogenic Escherichia coil was isolated from a died swine.It was indentified by biochemical test,toxicity experiment and drug sensitivity test and PCR amplification and sequencing.Drug sensitivity test showed that the Escherichia coil were only highly sensitive to Azithromycin and Clindamycin,moderately susceptible to Doxycycline,Polyadherin B and resistant to Bacitracin,Chloramphenicol,Polyadherin E and the others.The study showed that the isolated Escherichia coil strain has certain pathogenicity and resistant to many antibiotics which causes the swine death.

关 键 词：分离 鉴定 大肠杆菌 药敏试验 

分 类 号：S852.612[农业科学—基础兽医学]