高粱属牧草分子指纹图谱构建及遗传聚类分析  

Construction of Molecular Fingerprint Pattern and Genetic Clustering Analysis in Sorghum Forage

作　　者：陈超[1] 张宇君[1] 赵丽丽[1] 王普昶 申记 唐华江 CHEN Chao;ZHANG Yu-jun;ZHAO Li-li;WANG Pu-chang;SHEN Ji;TANG Hua-jiang(Department of Grass Science, College of Animal Science, Guizhou University, Guizhou Guiyang 550025 , China;Guizhou Pratacultural Institute, Guiyang Guizhou 550006,China;Station of Forage Grass and Fodder, Yinjiang Bureau of Agriculture and Animal Husbandry Science and Technology, Guizhou Yinjiang 555200, China)

机构地区：[1]贵州大学动物科学学院草业科学系,贵州贵阳550025 [2]贵州省草业研究所,贵州贵阳550006 [3]贵州省印江土家族苗族自治县农牧科技局饲草饲料工作站,贵州印江555200

出　　处：《西南农业学报》2017年第10期2191-2195,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家科技支撑计划(2014BAD23B03);国家自然科学基金(31560664);贵州省科技计划项目[黔科合NY字(2014)3048];黔科合支撑[(2016)2516]

摘　　要：【目的】为建立快速有效的高粱属牧草品种鉴定分析方法。【方法】采用基于DNA混池的ISSR分子标记技术,遵循多态性高、稳定性强的原则,构建高粱(Sorghum bicolor)、甜高粱(Sorghum dochna)、苏丹草(Sorghum Sudanense)、高丹草(Sorghum bicolor×S.sudanense)4个高粱属牧草共12个品种的数字指纹,并进行鉴定分析和聚类分析。【结果】利用DNA混池技术从110个ISSR引物中筛选出13个多态性高、重复性好的扩增引物,计算出12个高粱属牧草品种的遗传相似系数在0.6867~0.9036。当Gs为0.786时,12个品种可以聚为4类,第一类群包括6个高丹草品种和2个高粱品种;第二类群为2个甜高粱品种;第三类群为苏丹草品种超级丹;第四类群为苏丹草品种布鲁赛。根据引物扩增位点的特异性,挑选出3条核心引物(引物S37结合S35和S50),构建出能区分12个高粱属牧草品种的ISSR数字指纹。【结论】利用ISSR分子标记技术能有效鉴定高粱属牧草品种的纯度、真伪,揭示品种间遗传差异。【Objective】This study aimed to establish the rapid and effective analysis method for identification of Sorghum forage varieties.【Method】The digital fingerprint,identification and clustering of 12 forage varieties of Sorghum bicolor,Sorghum dochna,Sorghum Sudanense and Sorghum bicolor × S. sudanense are respectively analyzed by the ISSR molecular marker technique based on the mixed DNA pool【Result】13 amplification products with high polymorphism and good repeatability are screened from 110 ISSR primers by ISSR. The genetic similarity coefficient of 12 forage varieties is 0. 6867-0. 9036. When Gs is 0. 786,12 forage varieties can be divided into four groups. There are six S. bicolor × S. sudanense varieties and two S. bicolor varieties in Group I,two S. dochna varieties in Group Ⅱ,one S. sudanense variety in Group Ⅲ and one S. sudanense variety in Group Ⅳ. Three core primers（ S37,S35 and S50） are selected to differentiate ISSR digital fingerprint of 12 Sorghum forage varieties.【Conclusion】The ISSR molecular marker technique can effectively identify the purity and true or false of Sorghum forage varieties and reveal genetic difference among Sorghum forage varieties.

关 键 词：高粱属牧草 ISSR 数字指纹 聚类分析 

分 类 号：S431.14[农业科学—农业昆虫与害虫防治]