紫背天葵遗传多样性的ISSR分析（英文）  

Genetic Diversity of Wild Begonia fimbristipulata Hance Revealed by ISSR Analysis

作　　者：赵博[1,2] 毛世忠[1] 李景剑[1] 黄仕训[1] 钟树华[1] ZHAO Bo;MAO Shi-zhong;LI Jing-jian;HUANG Shi-xun;ZHONG Shu-hua(Guangxi Institute of Botany, The Chinese Academy of Sciences, Guangxi Guilin 541006 , China;Institute of Chinese Materia Medi- ca, China Academy of Chinese Medical Sciences, Beijing 100700, China)

机构地区：[1]中国科学院广西植物研究所,广西桂林541006 [2]中国中医科学院中药研究所,北京100700

出　　处：《西南农业学报》2017年第10期2224-2229,共6页Southwest China Journal of Agricultural Sciences

基　　金：基金项目:广西秋海棠属(秋海棠科)植物DNA条形码技术应用(2012GXNSFBA053075)

摘　　要：【目的】对野生紫背天葵资源的遗传多样性研究可为其育种、利用和保护提供参考依据。【方法】应用ISSR分子标记技术对广东肇庆鼎湖山、广西桂林大野神境、青狮潭3个紫背天葵野生居群的遗传多样性进行分析。采用POPGEN32进行数据分析,UPGMA绘制聚类图。【结果】12条ISSR引物共检测到112个清晰的扩增位点,多态性位点102个,多态位点百分率为91.07%;Nei's基因多样性指数(He)为0.3383,Shannon多样性指数(I)为0.5011,基因分化系数(Gst)为0.1507。居群间的遗传相似系数为0.8949~0.9162,平均为0.9026。聚类分析可知,各居群个体的聚类与其地理位置一致。【结论】紫背天葵具有较高的遗传多样性,但居群间的遗传分化水平较低,建议可将各种群后代种子或幼苗相互交叉移栽,以提高群体间的基因交流,增加遗传多样性水平。【Objective】Genetic diversity of wild Begonia fimbristipulata Hance would offer practical advices for its further breeding,utilization and conservation. 【Method】To assess the genetic diversity of B. fimbristipulata Hance in 3 wild populations,the molecular marker technique ISSR was used to investigate the genetic diversity of 55 samples from 3 populations in Guangdong Zhaoqing dinghusha,Guangxi Guilin Dayeshenjing and Liutang Qingshitan. Data was analyzed by POPGEN32,and a cluster diagram was presented by UPGMA. 【Result】One hundred and twelve amplified fragments were obtained using 12 ISSR primers. 102 polymorphic loci were identified,with a percentage of 91. 07 %. Nei＇s genetic diversity index（ He） was 0. 3383,Shannon diversity index（ I） was 0. 5011,and the coefficient of gene differentiation（ Gst） was 0. 1507. The genetic similarity coefficient among the populations ranged from 0. 8949 to 0. 9162 in an average of 0. 9026.Based on the clustering analysis,the individuals of each population group together related to geographic distribution. 【Conclusion】B. fimbristipulata Hance holds high genetic diversity and the majority of genetic variation occurs in the population with a low level of genetic differentiation among populations. In order to increase the genetic diversity,we suggested that seeds or seedlings collected from each wild populations and cross-breeding.

关 键 词：紫背天葵 ISSR分子检测 遗传多样性 

分 类 号：Q948[生物学—植物学]