植物生长物质和水解乳蛋白对蓖麻再生体系建立的影响  

Effects of Plant Growth Substances and Hydrolyzed Milk Protein on Establishment of Castor Regeneration System

作　　者：杜雪玲[1] 余如刚[1] 宋运贤[1] 张慧君[1] 

机构地区：[1]淮北师范大学生命科学学院,安徽省资源植物学重点实验室,安徽淮北235000

出　　处：《淮北师范大学学报（自然科学版）》2016年第4期69-74,共6页Journal of Huaibei Normal University：Natural Sciences

基　　金：2014安徽省资源植物学重点实验室项目

摘　　要：为筛选蓖麻再生体系建立的最佳配方,本研究以云杂N-2蓖麻种子为供试材料,对蓖麻种子消毒后,取胚轴分别接种在MS(Murashige and Skoog)为基本培养基上,并附加不同浓度的IAA(0.1 mg/L、0.3 mg/L和0.5 mg/L)、6-BA(0.5 mg/L、0.7 mg/L和0.9 mg/L)及不同浓度水解乳蛋白(0 mg/L、250 mg/L、500 mg/L和750 mg/L),进行愈伤组织培养和分化培养.结果表明:在IAA浓度为0.3 mg/L、6-BA浓度为0.7 mg/L时蓖麻愈伤组织诱导率最高为80%;在IAA和6-BA浓度最适,水解乳蛋白的浓度为500 mg/L时,愈伤组织生长状况最佳,呈淡黄色,褐化程度低;诱导芽增殖的最适培养基为MS+0.5 mg/L 6-BA+0.02 mg/L NAA,芽的增殖系数达6.15.To screen the best formula of the castor callus regeneration system,the seeds of castor(N-2)wereused as explants for in vitro regeneration.After surface sterilization of seed,the hypocotyl explants were cul-tured on Murashige and Skoog(MS)basal medium,supplemented with IAA(0.1,0.3 and 0.5 mg/L),6-BA(0.5,0.7 and 0.9 mg/L)and hydrolyzed milk protein(250,500 and 750 mg/L)for induction,culture and dif-ferentiation of callus.As a result,the formula of MS+ IAA 0.3 mg/L+ 6-BA 0.7 mg/L was the highest in cal-lus induction rate(80%)of castor.Based on this result,In hydrolyzed milk protein(500 mg/L),the growthof calli were the best,which were shown pale yellow and soft,and lower browning degree.In addition,the bestformula of propagation was MS+ 6-BA 0.5 mg/L+ IAA 0.02 mg/L,and the multiplication coefficient was6.15.

关 键 词：蓖麻 植物生长物质 水解乳蛋白 愈伤组织 芽增殖 

分 类 号：S565.6[农业科学—作物学]