杂交水稻恢复系福恢7185 OsSDR1基因的克隆与序列分析  

Cloning and Sequence Analysis of OsSDR1 Gene from Restorer Line of Hybrid Rice Fuihui 7185

作　　者：许惠滨[1,2] 徐倩[1,2,3] 连玲[1,2] 朱永生[1,2,3] 蒋家焕[1,2] 谢鸿光[1,2] 罗曦 江敏榕[1,2] 郑燕梅[1,2,3] 谢华安 张建福 

机构地区：[1]福建省农业科学院水稻研究所,福建福州350019 [2]农业部华南杂交水稻种质创新与分子育种重点实验室/福州(国家)水稻改良分中心/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室/福建省作物种质创新与分子育种省部共建国家重点实验室培育基地/杂交水稻国家重点实验室华南研究基地/水稻国家工程实验室,福建福州350003 [3]福建农林大学,福建福州350002

出　　处：《福建农业学报》2017年第9期939-945,共7页Fujian Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31501387);福建省自然科学基金项目(2015J05060);杂交水稻国家重点实验室开放课题(2016KF11);福建省农业科学院青年英才计划项目(YC2015-14)

摘　　要：植物种子从休眠到萌发的转变是其生命周期中一个关键的生理过程,其中,植物生长调节剂发挥了重要的调控作用,而SDR1(short-chain dehydrogenase/reductase 1)是调控营养信号转导和植物生长调节剂合成的关键酶。本研究克隆获得了福恢7185、福恢653和云恢290中SDR1基因的CDS序列,比对发现其在3个不同休眠品种并无差异,但与NCBI上日本晴的基因序列相比,缺失了9个碱基,3个丙氨酸;结合qRT-PCR技术分析SDR1基因在不同休眠水稻品种种胚中的表达情况,探索其与种子休眠的关系,同时利用GST pull-down技术找到SDR1的1个互作蛋白,结果表明,该蛋白与休眠相关,初步表明SDR1基因可能通过与其它结构原件的互作来调控种子休眠。The transition from dormancy to germination in seeds is a key physiological process during the lifecycle of plants.Hormones play an important regulatory role on it,While,short-chain dehydrogenase/reductase（SDR1）is a molecular link between nutrient signaling and plant hormone biosynthesis.The coding sequences（CDS）of SDR1 were cloned from different dormancy varieties FH7185,FH653 and YH290,respectively.The results of sequences blast indicated that the coding regions of OsSDR1 among the three varieties were homologous,but there were 9 bases deletion,coding three alanine compared to Nipponbare. Meanwhile,the results of real-time quantitative PCR（qRT-PCR）of SDR1 expression level in seed embryo among different dormancy varieties indicated that the expression level of SDR1 was significantly different among them.Moreover,the corresponding interaction proteins of OsSDR1 were screened by GST pull-down experiments and an interaction protein of OsSDR1 was found and its Genbank serial number designated as Os05 g0140800,it was verified related to seed dormancy.The preliminary results showed that SDR1 gene might regulate seed dormancy through interaction with other structural elements.

关 键 词：水稻 福恢7185 OsSDR1 克隆 序列分析 互作蛋白 

分 类 号：S511[农业科学—作物学]