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机构地区:[1]云南省农业科学研究院生物技术与种质资源研究所,云南昆明650223 [2]云南中烟工业有限责任公司技术中心,云南昆明650106 [3]云南省烟草公司昭通市公司,云南昭通657000 [4]云南省烟草公司丽江市公司,云南丽江674100
出 处:《福建农业学报》2017年第9期987-995,共9页Fujian Journal of Agricultural Sciences
基 金:中国烟草总公司云南省公司科技计划项目(2015YN21);云南中烟工业公司科技项目(2015CP08)
摘 要:为研究不同香型烟叶中生物碱的差异规律,运用经典的抽样方法,从国内15个省份中的71个市和县收集2011-2013年间3类香型烤烟烟叶共543个样本。根据现行相关的行业标准文献方法进行定量检测,并采用F-假设检验、Q-型聚类分析和判别分析等数理统计手段研究了不同香型烟叶中生物碱含量的差异规律。结果表明:2011年和2012年度3种香型烟叶中新烟草碱和2,3'-联吡啶含量差异水平较为相似,可以用来区分不同香型烟叶;烟叶中假木贼碱是2013年度不同香型的特征物质,可以用来区分清香和浓香型烟叶。不同年度3种香型中6种生物碱物质的含量均可以聚成3类;采用烟碱、降烟碱、麦斯明、假木贼碱、新烟草碱和2,3'-联吡啶等较少数的成分指标的含量来对3类香型烟叶样本进行模式识别和判定,回判及预测正确率分别为55.7%~71.6%、50.0%~69.8%。Alkaloids in tobaccos of different aromatic classifications were compared.Standard sampling method was employed to collect 543 flue-cured tobacco leaf specimens from 71 cities/counties in 15 provinces in China during 2011 and 2013.The samples were analyzed using the current industry methods,F-hypothesis testing,Q-clustering and discriminant analyses,as well as statistic methodology.The results showed that,between 2011 and 2012,the differences on the contents of and 2,3'-bipyridyl in the 3 categories of tobaccos were similar,and could be used for differentiating the varieties;while anabaseine found in 2013 was a characteristic substance that could be used to distinguish between the lightly and the strongly aromatic types of tobaccos.In those years,the 6 alkaloid compounds tested in the 3 categories of tobaccos could be clustered into 3 groups.By using the contents of nicotine,reduced nicotine, Mai Siming,anabaseine,,and 2,3'-bipyridine as indicators,the 3 aromatically different classifications of tobaccos could be identified with a discrimination rate of 55.7-71.6% and an accuracy of 50.0-69.8%.
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