骨内单次注射小剂量辛伐他汀对大鼠心梗后血管新生的影响  

Effect of single intraosseous injection of simvastatin on neoangiogenesis in myocardial infarction in rats

作　　者：刘灿[1] 海宝[1] 张稳[1] 祝俊雄 王红[1,2] 李子健 徐迎胜[4] 宋纯理[1,2] 

机构地区：[1]北京大学第三医院骨科,北京100191 [2]脊柱疾病研究北京市重点实验室,北京100191 [3]北京大学第三医院心内科,北京100191 [4]北京大学第三医院神经内科,北京100191

出　　处：《中国实验动物学报》2017年第5期506-512,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(NO.81672133;81641079);国家高技术研究发展计划(863计划NO.2015AA020304)

摘　　要：目的研究骨内局部单次注射小剂量辛伐他汀对大鼠心梗后血管新生和心功能的影响。方法Wistar大鼠随机分为假手术组、心肌梗死模型组和骨内注射辛伐他汀组(n=12)。冠状动脉左前降支结扎建立大鼠心肌梗死模型。24 h后实验组左胫骨内单次注射辛伐他汀0.5 mg,4周后分别通过小动物超声心动图评价左室功能,三苯基氯化四氮唑(TTC)染色计算心肌梗死面积,免疫荧光染色检测局部血管新生情况。结果超声心动图结果表明心肌梗死4周后左心室收缩功能明显下降,骨内注射辛伐他汀对大鼠心肌梗死后左心室功能未见明显改善;TTC染色发现骨内注射辛伐他汀组心肌梗死面积未见明显减少;免疫荧光染色显示,骨内注射辛伐他汀组心肌血管密度没有显著增加。结论大鼠心梗24 h后骨内单次注射小剂量辛伐他汀(0.5 mg),心肌梗死面积、血管新生及心脏功能无显著改善。Objective To explore the effect of single local intraosseous injection of small dose simvastatin on the angiogenesis and cardiac function in rats after myocardial infarction. Methods Adult male Wistar rats were divided into sham operation group,myocardial infarction model group and intraosseous injection of simvastatin 0. 5 mg group（ all n = 12 per group）. The left anterior descending branch of coronary artery was ligated to establish a rat model of myocardial infarction. The left ventricular function was evaluated by small animal echocardiography at 4 weeks postoperatively. The rest of the rats were sacrificed,the myocardial infarct size was evaluated by TTC staining,and the myocardial neovascularization was detected by immunofluorescence staining. Results We successfully established the rat model of myocardial infarction.The echocardiography showed that the left ventricular systolic function was decreased significantly at 4 weeks after myocardial infarction. Intraosseous injection of simvastatin（ 0. 5 mg） did not improve the left ventricular function after myocardial infarction in the rats. TTC staining showed that intraosseous injection of simvastatin did not reduce myocardial infarct size.Immunofluorescence staining showed that the myocardial capillary density of simvastatin group was slightly higher than thatof myocardial infarction model group,but showing no significant difference between them. Conclusions Intraosseous injection of simvastatin 0. 5 mg 24 hours after myocardial infarction cannot significantly promote myocardial angiogenesis,which is believed to be beneficial to the revascularization after ischemia,and thus failed to improve the cardiac function.

关 键 词：骨内注射 辛伐他汀 血管新生 心肌梗死 内皮祖细胞 

分 类 号：Q95-33[生物学—动物学]