电针对椎间盘脱出模型犬体感诱发电位的影响  

Effect of electroacupuncture on somatosensory evoked potentials in dog models of intervertebral disc prolapse

作　　者：李拓[1] 孔学礼 丛心宇 朱玉欣[1,2] 姜代勋[1,2] 陈武[1,2] 

机构地区：[1]北京农学院动物科学技术学院,北京102206 [2]北京农学院兽医学(中医药)北京市重点实验室,北京102206

出　　处：《中国实验动物学报》2017年第5期519-523,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金项目(No.31372473);北京农学院学位与研究生教育改革与发展项目(No.5056516004/024)

摘　　要：目的研究电针对椎间盘脱出模型犬脊髓损伤的修复作用及其对体感诱发电位(somatosensory evoked potential,SEP)的影响。方法比格犬随机分为三组,模型组和电针组采用球囊压迫法制作椎间盘脱出模型,电针组术后每天电针治疗;对照组进行假手术处理。术前(0 d)和术后1、4、7、14 d每只犬均使用德克萨斯犬脊髓损伤Texas Spinal Cord Injury Scale for Dogs(TSCIS)评分法评分,使用肌电诱发电位仪测量SEP并分析其潜伏期和波幅。结果术后1 d模型组和电针组与对照组TSCIS评分相比均显著降低(P<0.01),术后14 d,电针组与模型组相比差异有显著性(P<0.01);术后4 d模型组和电针组的SEP潜伏期相比显著降低(P<0.05),术后14 d,电针组与模型组的潜伏期相比差异有显著性(P<0.05);术后1 d模型组和电针组的SEP波幅与对照组相比显著降低(P<0.05),术后14 d,电针组与模型组的波幅相比差异有显著性(P<0.05)。结论电针能够有效促进椎间盘脱出模型犬的脊髓损伤修复,提高TSCIS评分,恢复SEP波形,缩短其潜伏期,提升其波幅;SEP能够在一定程度上反应脊髓损伤程度,评价电针治疗效果。Objective To study the effect of electroacupuncture on repair of spinal cord injury and its effect on somatosensory evoked potential（ SEP） in dog models of intervertebral disc prolapse. Methods Nine Beagle dogs were randomly divided into three groups. In the model group and electroacupuncture group,the dog disc prolapse models were made by balloon compression,and in the electroacupuncture group,electroacupuncture was used every day for 14 days after operation. The model group was not treated after surgery. Sham operation was performed in the control group. Each dog was scored according to the Texas Spinal Cord Injury Scale for Dogs（ TSCIS） scores before surgery（ day 0） and on days 1,4,7,14 after surgery. At the same time,SEP wave was measured using an EMG Evoked Potential Measuring Systerm and its latency and amplitude were analyzed. Results There was a significant difference in TSCIS scores between the model group,electroacupuncture group and control group at 1 day after operation. There was a significant difference between the electroacupuncture and model groups at 14 days after surgery. The amplitude of SEP in the model and electroacupuncture groups was significantly different from that in the control group at 1 day after operation,and there was a significant difference between the electroacupuncture and model groups at 14 days after operation. There was a significant difference in the latency of SEP between the model and electroacupuncture groups at 4 days after operation,and between the electroacupunc-ture and model groups after at 14 days after operation. Conclusions Electroacupuncture can effectively promote healing of spinal cord injury in dogs with intervertebral disc prolapse,improve the TSCIS scores,restore SEP waveform,shorten the latency and enhance the amplitude. SEP can reflect the degree of spinal cord injury to a certain extent,and can be used to evaluate the effect of electroacupuncture treatment in these dogs.

关 键 词：椎间盘脱出模型犬 电针 体感诱发电位 潜伏期 波幅 犬 

分 类 号：Q95-33[生物学—动物学]