豚鼠皮肤瘙痒动物模型的建立及评价  

Establishment and evaluation of a guinea pig model of itching

作　　者：米本中[1] 杨武斌[1] 罗晓玲[1] 王旖 胡娟[1] 徐嘉红[1] 樵星芳[1] 

机构地区：[1]重庆市中药研究院实验动物研究所,重庆400065

出　　处：《中国实验动物学报》2017年第5期528-533,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：重庆市科技项目(No.2015cstc-jbky-01907;cstc2015shmszx10010)

摘　　要：目的通过星点设计-效应平面法优化组胺和4-氨基吡啶(4-AP)联用建模的剂量,旨在建立一种新型的豚鼠瘙痒动物模型。方法用星点设计安排实验,各组分别在脱毛部位皮下注射0.5 m L不同致痒剂,记录豚鼠30 min内的搔抓潜伏期和搔抓次数,用综合评分法筛选最佳致痒组方。通过观察豚鼠的行为学变化和测定血液中的组胺和白介素-6(interleukin-6,IL-6)的含量对豚鼠模型进行评价。结果行为学实验发现,联合组在30 min内搔抓次数比组胺组和4-AP组均显著增多(P<0.01),搔抓潜伏期显著缩短(P<0.01)。4-AP组在30 min内搔抓次数比组胺组显著增多(P<0.01)。与对照组相比,联合组、组胺组豚鼠血清的组胺浓度均明显升高(P<0.05或P<0.01),但联合组升高程度低于组胺组(P<0.05);与对照组相比,组胺组、4-AP组和联合组的血清IL-6浓度均有显著的升高(P<0.01或P<0.05),且联合组明显高于组胺组和4-AP组。病理学检查显示,各模型组均出现不同程度的炎细胞增殖,其中联合组的反应更为明显。结论本试验中优化组方(联合组)模型容易实现并且有良好的重复性。Objective To optimize the optimal doses of histamine and 4-aminopyridine（ 4-AP） in the establishment of guinea pigs models of itching,and to establish a new guinea pig model of itching. Methods The central composite design-response surface method was used to arrange the experiment. In the experiment different pruritus agents were hypodermically injection of 0. 5 m L in the depilated area,and the scratching incubation period and scratching number in 30 minutes were counted after the injection. The guinea pig itching model was evaluated by observing the behavioral changes of guinea pigs and measuring the levels of histamine and interleukin-6 in the blood. Results The behavioral experiments found that the scratching frequency in the the combination group was significantly higher than the histamine group and 4-AP group（ P〈0. 01）. The itching latency of the combination group was significantly shorter than that of the histamine group and 4-AP group（ P〈0. 01）. Compared with the control group,the histamine concentrations of the combination group and histamine group were significantly increased（ P〈0. 05 or P〈0. 01）,and the level of the combination group was lower than that of the histamine group（ P〈0. 05）. Compared with the control group,the serum IL-6 concentrations of histamine group,4-AP group and combination group were significantly higher（ P〈0. 01 or P〈0. 05）,and those in the combination group were significantly higher than the histamine and 4-AP groups. Compared with the control group,pathologic examination showed proliferation of inflammatory cells in all model groups,and the reaction of the combination group was more obvious. Conclusions The optimal conditions used in this experiment are easy to achieve and have good reproducibility inthe establishment of a guinea pig model of itching.

关 键 词：组胺 4-氨基吡啶 豚鼠 瘙痒模型 

分 类 号：Q95-33[生物学—动物学]