^(68)Ge/^(68)Ga发生器的临床应用  

Application of the ^(68)Ge/^(68)Ga Generator in Clinic

作　　者：黄钱焕 潘栋辉[2] 徐宇平[2] 盛洁 王彦婷 杨敏 

机构地区：[1]南京医科大学第一临床医学院,江苏南京210029 [2]江苏省原子医学研究所卫生部核医学重点实验室江苏省分子核医学重点实验室,江苏无锡214063

出　　处：《同位素》2017年第4期270-275,共6页Journal of Isotopes

基　　金：国家自然科学基金(51473071;81472749;31671035);江苏省卫计委创新团队资助项目(CXTDA2017024)

摘　　要：对新型^(68)Ge/^(68)Ga发生器的最佳淋洗时间、最佳峰段以及不同缓冲液对68 Ga显像及生物分布的影响进行研究,为动物实验和临床应用提供参考。采用4mL 0.05mol/L HCl淋洗740 MBq ITG^(68)Ge/^(68)Ga发生器,首次淋洗后分别隔2、4、6、8、10、12、14h再次淋洗,记录放射性活度;先以每段0.5mL收集淋洗液,在比活度较高段以每0.1mL收集淋洗液,记录放射性活度;分别用乙酸钠、NaOH、4-羟乙基哌嗪乙磺酸(HEPES)调节^(68)Ga^(3+)溶液pH,在正常小鼠体内行microPET显像。结果表明:在淋洗4h后,放射性活度可达平衡时的91%;最佳峰段为2~3 mL,放射性活度峰段的0.1 mL可收集22 MBq的^(68)Ga^(3+);^(68)Ga^(3+)在血液中摄取值高,1h仍有6.9%ID/g,表明游离的^(68)Ga^(3+)可能会影响图像的对比度,需要在标记放射性药物后去除残余的游离^(68)Ga^(3+);膀胱的放射性摄取高,提示^(68)Ga^(3+)通过泌尿系统排泄;乙酸钠、NaOH、HEPES调节pH后对^(68)Ga在正常小鼠体内显像及生物分布均无统计学差异(P>0.05)。新型^(68)Ge/^(68)Ga发生器操作简便,每4h即可淋洗一次,淋洗液的峰段在2~3mL,建议临床采用乙酸钠缓冲液。ITG ^68Ge/^68Ga generator allows for dilute HCl to effectively elute ^68Ga^3＋without metal impurities.The impact of the optimal elution time,the best peak segment and the effects of different buffers on the imaging and biodistribution of the 68 Ga were explored with the ITG^68Ge/^68Ga generator.According to the manufacturer protocol,a740 MBq self-shielded ^68Ge/^68Ga generator was eluted with 4 mL of 0.05 mol/L HCl.After the beginning of elution,the generator was eluted again at 2,4,6,8,10,12,14 hours to record radioactivity.The 68 Ga was eluted with HCl in each fraction of0.5 mL,then eluted with every 0.1 mL in the dense fractions and the radioactivity was recorded.MicroPET imaging and biodistribution were performed in normal mice after adjusting the pH of ^68Ga^3＋solution with sodium acetate,NaOH and HEPES separately.The results showed that 4 hours post elution,91% of the maximum achievable radioactivity was accumulated.The best peak was at 2-3 mL and the radioactivity of the top fraction（0.1 mL）was 22 MBq.The uptake of ^68Ga^3＋radioactivity was 6.9%ID/g in the blood at 1 hour post injection,suggesting that purity of a tracer was essential because free ^68Ga^3＋may affect the contrast of the image and dection of tumors.The radioactivity uptake of the bladder was high,suggesting that the ^68Ga^3＋was metabolized by the urinary system.There was no significant difference in the ^68Ga^3＋imaging and biodistribution as using sodium acetate,HEPES and NaOH as buffer solution（P0.05）.The results showed that ITG^68Ge/^68Ga generator was simple and convenient for manual operation.The generator could be eluted every 4 hours and the best peak segment was at 2-3 mL.Sodium acetate seems to be the most appropriate buffer in clinic.

关 键 词：^68Ge/^68Ga发生器 缓冲液 正电子发射断层扫描显像(PET) 生物分布 

分 类 号：TL364.5[核科学技术—核技术及应用]