HPLC法测定红曲灵芝丹参胶囊中4种成分的含量  

Determination of 4 components in Monascus Ganoderma Salvia Capsules by HPLC

作　　者：曾敏[1] 王雨萌 贾璞[1] 王玥 吴妮 刘颖 王洁[1] 于洁[1] 白亚军[1] 邢连喜[1] 郑晓晖[1,2] 

机构地区：[1]西北大学生命科学院,西安710069 [2]深圳清华大学研究院,深圳518057

出　　处：《西北药学杂志》2017年第6期679-682,共4页Northwest Pharmaceutical Journal

基　　金：陕西省中医管理局中医药科研课题(编号:13-JC030);陕西省重点科技创新团队计划(编号:2013KCT-24);2015年教育部"创新团队发展计划"滚动支持(编号:IRT-15R55);西北大学校级大学生创新创业训练(编号:2016183);西北大学校级大学生创新创业训练(编号:2017156)

摘　　要：目的建立同时测定红曲灵芝丹参胶囊与丹参提取物中丹参素钠、原儿茶醛、丹酚酸B和丹参酮ⅡA含量的HPLC法。方法采用Agilent TC-C18(250mm×4.6mm,5μm)色谱柱;流动相为2 mL·L^(-1)甲酸水(A)-甲醇(B),梯度洗脱;流速为0.6mL·min^(-1);柱温:30℃;检测波长:280nm;进样量:20μL。结果测定红曲灵芝丹参胶囊中丹参素钠、原儿茶醛、丹酚酸B和丹参酮ⅡA 4种成分的含量,每1g中丹参素钠、原儿茶醛、丹酚酸B和丹参酮ⅡA的含量分别为0.451 2,0.119,1.398和3.789mg,与丹参提取物中各成分的含量相比差异较大。结论该方法简便、准确,灵敏度高,专属性好,可用于红曲灵芝丹参胶囊与丹参提取物的质量控制。Objective To establish an HPLC method for the simultaneous determination of salvianic acid A,protocatechualdehyde,salvianolic acid B and tanshinoneⅡA in Monascus Ganoderma Salvia Capsules and Salvia extract.Methods The HPLC separation was performed on an Agilent TC-C18（250 mm×4.6 mm,5μm）column at 30 ℃.The mobile phases consisted of methanol 2 mL·L^-1 formic acid solution with gradient elution at a flow rate of 0.6 mL·min^-1.The UV detection wavelength was 280 nm,and the injection volume was 20μL.Results In 1 g of the powder of Monascus Ganoderma Salvia Capsules,the contents of salvianic acid A,protocatechualdehyde,salvianolic acid B and tanshinone ⅡA were 0.451 2,0.119,1.398 and 3.789 mg,respectively.Compared with the content of each component in the salvia miltiorrhiza extract,the contents in the capsules were about a quarter.Conclusion The method was validated to be simple,accurate,selective,and sensitive,which can be used for the quality control of Monascus Ganoderma Salvia Capsules and Salvia miltiorrhiza extract.

关 键 词：红曲灵芝丹参胶囊 丹参素钠 原儿茶醛 丹酚酸B 丹参酮ⅡA 

分 类 号：R927.2[医药卫生—药学]