复黄片总鞣质、总黄酮及槐角苷的含量测定  

Determination of total tannins,total flavonoids and sophoricoside in Fuhuang Tablets

作　　者：张彪[1] 马欣 潘一滨[2] 苏靖 安红梅[2] 聂曲 邱明丰[1,3] 

机构地区：[1]上海交通大学药学院,上海200240 [2]上海中医药大学附属龙华医院,上海200032 [3]滇西应用技术大学傣医药学院,景洪600100

出　　处：《西北药学杂志》2017年第6期740-744,共5页Northwest Pharmaceutical Journal

基　　金：上海市科学技术委员会生物医药领域支撑项目(编号:14401900100)

摘　　要：目的建立复黄片中总鞣质、总黄酮及槐角苷的含量测定方法。方法以没食子酸和槐角苷为对照品,UV法测定复黄片中总鞣质及总黄酮的含量,检测波长分别为760和260nm。HPLC法测定复黄片中槐角苷的含量,采用Agilent TC-C18(2)色谱柱(250mm×4.6mm,5μm);柱温为30℃;流动相为乙腈-磷酸缓冲盐,采用梯度洗脱;流速为1.0mL·min-1;检测波长为254nm。结果复黄片中总鞣质、总黄酮及槐角苷的线性范围分别为1.04~5.20(r=0.999 5),4.15~9.34(r=0.999 5)和21.34~74.69μg·mL^(-1)(r=0.999 4),加样回收率分别为99.62%,100.20%和100.70%,平均含量分别为23.81,133.02和9.99mg·片^(-1)。结论该方法简便易行,结果准确、可靠,可用于控制复黄片的质量。Objective To establish methods for the determination of total tanins,total flavonoids and sophoricoside in Fuhuang Tablets.Methods The contents of total tanins and total flavonoids in Fuhuang Tablets were determined by UV with gallic acid and sophoricoside as reference.The detection wavelengths were 760 and 260 nm.The separation of sophoricoside was performed on an Agilent TC-C18（2）column（250 mm×4.6 mm,5μm）at 30 ℃ with gradient elution using phosphate buffer and acetonitrile as mobile phase at a flow rate of 1.0 mL·min^-1.The detection wavelength was 254 nm.Results Linear ranges of total tanins,total flavonoids and sophoricoside were 1.04-5.20（r=0.999 5）,4.15-9.34（r=0.999 5） and 21.34-74.69μg·mL^-1（r=0.999 4）,respectively.The average recoveries of accuracy tests were 99.62%,100.20% and 100.70%,respectively.Contents of total tannins,total flavonoids and sophoricoside in Fuhuang Tablet were 23.81,133.02 and 9.99 mg·tablet-1,respectively.Conclusion The methods are simple and accurate.It can be used for the quality control of Fuhuang Tablets.

关 键 词：复黄片 UV HPLC 总鞣质 总黄酮 槐角苷 

分 类 号：R927.2[医药卫生—药学]