1、3、5月龄BALB/c小鼠的外周血与脾脏BCR H-CDR3组库的构成  

The Composition of BCR H-CDR3 Repertoires in Peripheral Blood and Spleen from 1, 3, 5 Months Old BALB/c Mice

作　　者：李月红[1] 潘雨蓉 孙素红[2] 马龙[1] 石彬[3] 马锐[1] 姚新生[1] 

机构地区：[1]遵义医学院免疫学教研室贵州省免疫学研究生教育创新基地,遵义563000 [2]遵义医学院附属医院甲乳外科,遵义563000 [3]遵义医学院检验医学系,遵义563000

出　　处：《中国细胞生物学学报》2017年第10期1304-1311,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:81660269)资助的课题~~

摘　　要：B细胞是机体适应性免疫的主要成分,是介导特异性体液免疫应答的主要细胞群,主要依靠B细胞受体(B cell receptor,BCR)与抗原发生应答反应。BCR的可变区为互补决定区(complementarity determining region,CDR)包括CDR1、CDR2、CDR3,其中CDR3具有最丰富的多样性。该文通过高通量测序并分析1、3、5月龄小鼠BCR重链(heavy chain,H)-CDR3组库的多样性、CDR3区的基因/氨基酸的组成特性和各亚家族的偏向取用等,结果发现,小鼠在发育过程中,其外周免疫器官(脾脏)和外周血BCR H-CDR3组库存在相应的动态变化。该研究探索了机体在发育过程中,BCR H-CDR3组库的构成变化,为病理条件下BCR H-CDR3组库的研究提供基础数据。B cell is the main component of adaptive immunity and the main cell group mediating specific humoral immune response. It mainly depends on the B cell receptor（BCR） to take part in antigen response. The variable region of B cell receptor is a complementary determinant region（CDR）, consisting of CDR1, CDR2, CDR3, among them, CDR3 has the most abundant diversity. This article through high-throughput sequencing analyzes BCR heavy chain（H）-CDR3 repertoire diversity, composition characteristics of CDR3 region gene or amino acids and bias usage in each subfamily gene of 1, 3, 5 months old mice, finds that BCR H-CDR3 repertoires of peripheral immune organs（spleen） and peripheral blood exist the corresponding dynamical change during the development of mice. The results of this study are helpful to explore the changes of BCR H-CDR3 repertoire during the development of organism and to provide basic data for the study of BCR H-CDR3 repertoire under pathological conditions.

关 键 词：BALB/C小鼠 B细胞受体重链-CDR3组库 发育 

分 类 号：R392[医药卫生—免疫学]