青海蚕豆中原花青素和左旋多巴的含量测定和品种间差异的比较  

Determination of Oligomeric Proanthocyanidins and Levodopa in Broad Bean from Qinghai Province and Comparison of Differences Among Varieties

作　　者：申士富[1,2] 钱静 刘廷[1,2] 李俊松 赵永贵[3] 狄留庆 SHEN Shi-fu QIAN Jing LIU Ting LI Jun-song ZHAO Yong-gui DI Liu-qing(College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China Jiangsu Engineering Research Center for Efficient Delivery System of Chinese Materia Medica, Nanfing 210046, China Qinghai Yuanxing Industry ＆ Trade Co. , Ltd., Xining 810000, China)

机构地区：[1]南京中医药大学药学院,南京210046 [2]江苏省中药高效给药系统工程技术研究中心,南京210046 [3]青海源兴工贸有限公司,西宁810000

出　　处：《中国食物与营养》2017年第9期36-40,共5页Food and Nutrition in China

基　　金：江苏省重点研发专项资金项目(项目编号:BF2016004);青海省星火计划项目(项目编号:2014-NS-306);江苏高校优势学科建设工程资助项目(项目编号:PAPD);江苏省"青蓝工程"创新团队支持计划资助(项目编号:QLP)

摘　　要：目的:建立青海蚕豆中左旋多巴和原花青素含量测定方法,测量22批样品的含量。方法:采用紫外分光光度法测量原花青素的含量,检测波长为554nm。采用HPLC法检测蚕豆中L-多巴含量:色谱柱:汉邦ODS-2(250mm×4.6mm,5μm),流动相:甲醇-0.1mol/L冰醋酸溶液(5∶95);流速:0.8 mL/min;测定波长:280 nm;柱温:25/3℃;进样量:20μL。结果:原花青素在75~375μg/mL范围内线性良好、L-多巴在75~375μg/mL范围内线性良好。结论:青海蚕豆中左旋多巴和原花青素含量测定方法稳定可靠。【Objective】To establish a method for determination of levodopa and oligomeric proanthocyanidins（OPC） content for measuring 22 batches of samples.【Method】Detection wavelength of UV spectrophotometric method for OPC was 554 nm.HPLC method was established to detect the content of L-dopa in broad bean：column：hanbon ODS-2（250 mm＊4.6mm,5 μm）,mobile phase：methanol-0.1mol/L acetic acid solution（5∶95）;flow rate 0.8 mL/min;detection wavelength 280 nm;column temperature 25℃;sample size20μL.【Result】The results showed that the range of 75~375μg/mL was good linear for OPC and L-DOPA.【Conclusion】The two methods were stable and reliable.The content of the 20 batches of samples were determined,and the changes of the content before and after processing were compared.

关 键 词：青海蚕豆 左旋多巴 原花青素 紫外分光光度法 HPLC 

分 类 号：O657.32[理学—分析化学] TS214.9[理学—化学]