基于DNA条形码技术对苍南海域仔稚鱼的物种鉴定  

Identification of Several Fish Larvae Based on DNA Barcoding in the Investigated Waters of Cangnan

作　　者：李渊[1,2] 张丽艳 张然 宋普庆[1] 王良明[1] 张立[1] 林龙山[1] 

机构地区：[1]国家海洋局第三海洋研究所,福建厦门361005 [2]海洋赤潮灾害立体监测技术与应用国家海洋局重点实验室,上海200137 [3]福建海洋研究所,福建厦门361013

出　　处：《中国海洋大学学报（自然科学版）》2017年第12期72-79,共8页Periodical of Ocean University of China

基　　金：海洋赤潮灾害立体监测技术与应用国家海洋局重点实验室开放基金项目(MATHAB201603);双边国际事务合作-开展海峡两岸合作与交流项目(2200207)资助~~

摘　　要：采用DNA条形码技术对苍南海域冬季采集的仔稚鱼进行物种鉴定,以提高物种鉴定的准确性。所有仔稚鱼通过形态特征鉴定出10个形态种,但经条形码技术可鉴定出11个有效种。序列相似性结果显示11个形态种中10种可鉴定到种的水平,1种无法匹配到相应的物种。条形码间隙分析结果显示各物种的最小种间遗传距离均高于该物种的最大种内遗传距离,存在明显的条形码间隙。由系统发育树可以看出同一形态种的不同个体聚为一支系,各物种间能明显区分开。同时结合本次渔业资源现场调查结果,发现11种仔稚鱼中仅有5种的成鱼出现在本次调查中,剩余6种为新出现种类。可见,DNA条形码技术除了能提高仔稚鱼物种鉴别的准确性外,还能对物种多样性调查起到一定的补充作用。The investigated waters of Cangnan located in the south of Zhejiang province,where is an important spawning ground for many marine species.Fishery resources survey in this water was conducted by bottom trawling in the winter of 2016 and a large number of fish larvae were collected.To improve the accuracy of species identification,DNA barcoding based on COI gene was employed to identify fish larvae collected from the investigated waters.Ten morphospecies were identified based on morphological characteristics,while 11 species were identified by DNA barcoding analyses.After running blast searches in NCBI,a match of 98%~100% was found in 96.59% of all individuals,a match of 92%~98% was 2.27%,and 85%~92% was 1.14%.Results of sequence similarity showed that ten of 11 species were identified to the species level,while one species was unmatched.There would be a clear overlap（barcoding gap）between intraspecific and interspecific variabilities in each species,as the smallest interspecific divergences were well below 2%,but the largest intraspecific divergences reached above 2%.Different individuals were clustered together,and different species could be unambiguously identified from NJ tree.Results noted above indicated that there were 11 valid species identified from this survey at last.Compared with the survey results of fishery resources,only five fish larvae species were identified with adult fish species found in this survey,and the six remaining species were new types.The COI barcoding diversity was found to be high,with the exception of Harpadon nehereus（0.250±0.180）.It was vital that some protective measures be taken immediately to H.nehereus because of low COI barcoding diversity.It is thus clean that DNA barcoding is an effective tool for accurate species identification,and also could play a supplementary role in the species diversity survey.All entire dataset in this study could play an important role in survey of biodiversity and genetic diversity of Cangnan,and all results could provide reference for f

关 键 词：DNA条形码 生物多样性 仔稚鱼 序列相似性 条形码间隙 

分 类 号：Q174[生物学—水生生物学] S917.4[生物学—普通生物学]