真菌免疫调节蛋白FIP在烟草中的瞬时表达  

Transient Expression of Fungal Immunomodulatory Protein(FIP) in Tobacco Plants

作　　者：周波[1] 王悦 许亚男 刘雨晴[1] 孟大伟 金太成[1] 杨丽萍[2] 

机构地区：[1]吉林师范大学生命科学学院,四平136000 [2]吉林师范大学植物资源科学与绿色生产重点实验室,四平136000

出　　处：《分子植物育种》2017年第9期3497-3501,共5页Molecular Plant Breeding

基　　金：吉林省教育厅项目(吉教科合字[2015]第231号);国家青年科学基金项目(31301043)共同资助

摘　　要：当短时间内需要高水平的基因表达时,采用病毒载体介导的植物瞬时表达系统是具有一定优势的。本研究中我们构建了植物瞬时表达载体p35S-30B-FIP,并将表达载体转化农杆菌EHA105,建立了植物瞬时表达体系。利用一种瞬时表达体系的新型农杆菌侵染技术(根部真空侵染法)在烟草(Nicotiana tabatum L.)中进行真菌免疫调节蛋白(fungal immunomodulatory protein,FIP)的瞬时表达,并采用Trizal法提取植物总RNA进行了半定量RT-PCR检测。研究结果表明:外源蛋白FIP在烟草(N.tabacum)中获得了瞬时表达。本研究为新型农杆菌侵染技术的利用以及药用蛋白的生产奠定了实验基础。Plant transient expression by using virus-based vector is advantageous when high level of gene expression is desired within a short time. In this study, we have constructed plant transient expression vector p35S-30B-FIP and system, and then the expression vector was transformed into Agrobacterium EHA105. The fungal immunomodulatory protein（FIP） was expressed successfully by using a new inoculation technology of transient expression system（root vaccum inoculation） in tobacco（Nicotiana tabatum L.） plants, and the expression of foreign protein was detected by RT-PCR after using Trizal to extract plant total RNA. The results showed that the foreign protein（FIP） was transiently expressed in tobacco plants. This study provided experiment basis for the usage of new inoculation technology and the production of pharmaceutical protein.

关 键 词：烟草 瞬时表达体系 根部真空侵染 真菌免疫调节蛋白 

分 类 号：Q943.2[生物学—植物学]