Distinct transcription profile, of genes involved in carotenoid biosynthesis among six different color carrot （Daucus carota L,） cultivars  

作　　者：Jing Ma Zhisheng Xu Guofei Tan Feng Wang Aisheng Xiong 

机构地区：[1]State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

出　　处：《Acta Biochimica et Biophysica Sinica》2017年第9期817-826,共10页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the Natural Science Foundation of Jiangsu Province （No. BK20130027）, the Priority Academic Program Development of Jiangsu Higher Education Institutions Project （PAPD）, and the Program for New Century Excellent Talents in University （No. NCET-11-0670）.

摘　　要：Carotenoid, a group of lipophilic molecules, is widely distributed in nature, and is important for plant photosynthesis and photoprotection. In carrot taproot, different types of dominant carotenoid accumulation lead to yellow, orange, and red colors. In this study, six different carrot cultivars were used to simultaneously analyze carotenoid contents by high performance liquid chromatography. The expression levels of genes involved in carotenoid biosynthesis of carrot were also detected by real-time quantitative PCR. It was found that genes involved in xanthophyll formation were expressed at high levels in yellow carrot cultivars. However, these genes were expressed at low levels in orange carrot cultivars. The contents of α- and β-carotene accounted for a large pro- portion in total carotenoid contents in orange carrot cultivars. These results indicate that α-carotene accumulation and xanthophyll formation may be related to the expression levels of carotene hydro-xylase genes in carrot.Carotenoid, a group of lipophilic molecules, is widely distributed in nature, and is important for plant photosynthesis and photoprotection. In carrot taproot, different types of dominant carotenoid accumulation lead to yellow, orange, and red colors. In this study, six different carrot cultivars were used to simultaneously analyze carotenoid contents by high performance liquid chromatography. The expression levels of genes involved in carotenoid biosynthesis of carrot were also detected by real-time quantitative PCR. It was found that genes involved in xanthophyll formation were expressed at high levels in yellow carrot cultivars. However, these genes were expressed at low levels in orange carrot cultivars. The contents of α- and β-carotene accounted for a large pro- portion in total carotenoid contents in orange carrot cultivars. These results indicate that α-carotene accumulation and xanthophyll formation may be related to the expression levels of carotene hydro-xylase genes in carrot.

关 键 词：CAROTENOID carotene hydroxylase genes xanthophyll formation HPLC RT-qPCR CARROT 

分 类 号：Q756[生物学—分子生物学] S666.062[农业科学—果树学]