力竭运动应激对小鼠脾T、B淋巴细胞功能及钾离子通道Kv1.3表达的影响  

Effect of Exhaustive Exercise on T and B lymphocytes' Function and Expression of Kv1.3 in Spleen of Mice

作　　者：傅涛[1] 江伟[2] 刘智强 矫玮[3] 

机构地区：[1]天津体育学院健康与运动科学系,天津310008 [2]宁夏大学体育学院,宁夏银川750021 [3]北京体育大学康复系,北京100084

出　　处：《北京体育大学学报》2017年第9期42-47,共6页Journal of Beijing Sport University

基　　金：天津市创新团队资助项目

摘　　要：目的:观察力竭运动应激后小鼠脾脏T、B淋巴细胞钾离子通道表达的差异性,探讨力竭运动应激对T、B淋巴细胞的影响。方法:复制小鼠力竭模型后从脾脏中分离出T、B淋巴细胞,测定淋巴细胞转化功能,溶血空斑形成实验,流式细胞仪T淋巴细胞周期和分型,RT-PCR、Western blot测小鼠脾脏T、B细胞Kv1.3的表达。结果:小鼠脾脏T淋巴细胞Kv1.3 mRNA和蛋白质表达水平明显下调(P<0.05),小鼠脾脏B淋巴细胞Kv1.3 mRNA和蛋白质表达水平没有显著性差异(P>0.05);力竭运动应激小鼠T淋巴细胞功能受抑制(P<0.05),B淋巴细胞功能未受到明显影响(P>0.05)。结论:力竭运动应激导致小鼠脾脏T、B淋巴细胞Kv1.3 mRNA和蛋白质表达呈现差异性,进而影响淋巴细胞功能,提示力竭运动应激条件下细胞免疫功能的抑制可能与钾通道有关,为进一步研究运动应激导致免疫抑制的机制提供了新的思路。Purpose： This study aimed at observing different expressions of Kv1.3 of T and B lymphocytes in spleen of mice after exhaustive exercise,and exploring the effect of exhaustive exercise on T and B lymphocytes.Methods： T and B lymphocytes in spleen of mice were separated after exhaustive exercise; lymphocyte transformation test（ LTT）,plague forming cell（ PFC） test were performed to demonstrate their functions; RT-PCR,Western blot were performed to demonstrate the mRNA and protein expressions of Kv1.3 of T and B lymphocytes in spleen.Results： Compared with control group,the mRNA and protein expressions of Kv1.3 were significantly down-regulated in T lymphocyte of spleen after exhaustive exercise（ P 0.05）,and there were no significant differences of B lymphocyte after exhaustive exercise（ P 0.05）; transformation function of T lymphocyte was affected by exhausted exercise（ P 0.05） and that of B lymphocyte was not（ P 0.05）.Conclusion： Exhaustive exercise changes the mRNA and protein expressions of Kv1.3 of T and B lymphocytes in spleen of mice,affects the function of lymphocytes,which indicate that the inhibition of cellular immune function under exhaustive exercise stress may be related to potassium channels.This study provided a new idea for further research on the mechanism of exercise inducing immunosuppression.

关 键 词：力竭运动 应激 Kv1.3 免疫抑制 淋巴细胞 

分 类 号：G804.7[文化科学—运动人体科学]