大剂量补充亮氨酸对抗阻运动所致骨骼肌损伤的影响  

Effects of High-Dose Leucine Supplementation on Skeletal Muscle Damage Induced by Resistance Exercise

作　　者：夏志[1] 赵艳[1] 孟思进[2] 史仍飞[3] 尚画雨 王良峡 刘娟 苏全生[4] 

机构地区：[1]井冈山大学,江西吉安343009 [2]武汉体育学院健康科学学院,湖北武汉430079 [3]上海体育学院运动科学学院,上海200438 [4]成都体育学院运动医学系,四川成都610041

出　　处：《北京体育大学学报》2017年第10期56-62,共7页Journal of Beijing Sport University

基　　金：吉安市科技支撑计划项目;江西省体育局局管科研项目;江西省大学生创新创业训练计划项目

摘　　要：目的:探讨补充亮氨酸(Leu)对无训练史被试进行抗阻运动所致骨骼肌损伤的影响。方法:16名青年男性大学生据体重随机分为补充Leu组与安慰剂对照组,Leu和安慰剂组被试从测试日深蹲抗阻运动前3周直至运动后4 d分别以每千克体重150 mg剂量摄入150 m L Leu或糊精溶液。测试日各组被试以50%1RM负荷完成5组深蹲运动,每组20次,组间间歇2 min。深蹲运动前于肘正中静脉采集血样,测量大腿围、纵跳高度、膝关节活动幅度与肌肉酸痛程度,并于运动后即刻、24 h、48 h、72 h和96 h重复测试。ELISA法检测血清CK-MM、Mb与血浆IL-6,胶乳增强免疫比浊法检测血清CRP。结果:与安慰剂组被试相比,补充Leu显著降低了Leu组被试运动后延迟性肌肉酸痛水平、大腿中段周长及血清CK-MM、Mb、CRP与血浆IL-6含量,而深蹲1-RM值、纵跳高度及膝关节ROM组间差异则并无统计学意义。结论:1)每千克体重150 mg剂量Leu能够削弱抗阻运动所导致的骨骼肌损伤与疼痛,该效应可能与炎症反应的抑制有关,且推测Leu对骨骼肌蛋白质代谢平衡的调节作用在其中亦有贡献;2)该剂量Leu不能明显促进工作肌机能恢复。Objective：Study investigated the effect of leucine supplementation on muscle damage induced by resistance exercise in untrained people.Methods：Sixteen male untrained college students were randomly divided into leucine supplementation group and placebo control group according to their body weight,all of them were fed150 m L leucine or dextrin solution at a dose of 150 mg per kilogram body weight from three weeks before exercise to 4 days after exercise.These students performed 5 sets of 20 repetitions at 50%1RM squat,with 2 minutes rest between each set.Blood sample was taken from median cubital vein,and vertical jump performance,mid-thigh circumference,range of motion（ROM）of knee joint,and perception of muscle soreness were assessed before squat exercise,immediately after exercise,and 24h,48h,72h and 96h after exercise.The activity of CK-MM,concentration of serum Mb and plasma IL-6 were measured by ELISA,and the concentration of serum CRP was detected by latex-enhanced immunoturbidimetry.Results：Compared with placebo control group,the perception of DOMS,mid-thigh circumference,activity of serum CK-MM,concentration of serum Mb,CRP and plasma IL-6 of leucine supplementation group were significantly lower,while 1-RM squat,vertical jump performance and ROM of knee of two groups had no difference.Conclusions：1）Leucine supplementation with the dosage of 150 mg/kg can attenuate skeletal muscle damage and soreness induced by resistance exercise,which may be correlated with inhibition of inflammatory response,moreover,the regulation effect of leucine on protein metabolism balance in skeletal muscle is supposed to play certain role in this process;2）the supplementation with this dosage can not promote the functional recovery of working muscles.

关 键 词：亮氨酸 抗阻运动 运动性肌肉损伤 延迟性肌肉酸痛 炎症 

分 类 号：G804.2[文化科学—运动人体科学]