Electrophysiological responses of the rice striped stem borer Chilo suppressalis to volatiles of the trap plant vetiver grass (Vetiveria zizanioides L.)  

作　　者：LU Yan-hui LIU Kai ZHENG Xu-song Lü Zhong-xian 

机构地区：[1]State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R. China

出　　处：《Journal of Integrative Agriculture》2017年第11期2525-2533,共9页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31672050);the National Key Research and Development Program of China(2016YFD0200800-04);the Zhejiang Key Research and Development Program,China(2015C02014)

摘　　要：Vetiver grass （Vetiveria zizanioides L.） was previously found to effectively attract female adults of Chilo suppressalis （Walker）, an important pest of rice. To determine the volatile compounds involved in this attraction, electroantennography （EAG） responses to seven synthetic volatiles released from vetiver plants were examined. Our results indicated that the responses of C. suppressalis adult antennae to the different compounds varied widely. The compounds elicited strong EAG responses in female antennae were subsequently selected for further EAG response tests, namely, caryophyllene, β-ocimene, linalool and a-pinene. EAG responses to a combination of these four compounds did not differ significantly from the individual compounds. However, pair combination tests indicated that 0.01μg μL-1linalool and 50 μg μL-1 α-pinene, 50 μgμL-1 caryophyllene and 0.01μg μL-1 linalool, 0.01 μg μL-1 13-ocimene and 0.01 μg μL-1 linalool, and 0.01μg μL-1 β-ocimene and 50 μg μL-1 caryophyllene elicited significantly greater EAG responses in 3-day female moths compared to the 1-day female. These compound combinations and the corresponding ratios are probably playing an important role in attracting female adults of C. suppressalis to the vetiver grass.Vetiver grass （Vetiveria zizanioides L.） was previously found to effectively attract female adults of Chilo suppressalis （Walker）, an important pest of rice. To determine the volatile compounds involved in this attraction, electroantennography （EAG） responses to seven synthetic volatiles released from vetiver plants were examined. Our results indicated that the responses of C. suppressalis adult antennae to the different compounds varied widely. The compounds elicited strong EAG responses in female antennae were subsequently selected for further EAG response tests, namely, caryophyllene, β-ocimene, linalool and a-pinene. EAG responses to a combination of these four compounds did not differ significantly from the individual compounds. However, pair combination tests indicated that 0.01μg μL-1linalool and 50 μg μL-1 α-pinene, 50 μgμL-1 caryophyllene and 0.01μg μL-1 linalool, 0.01 μg μL-1 13-ocimene and 0.01 μg μL-1 linalool, and 0.01μg μL-1 β-ocimene and 50 μg μL-1 caryophyllene elicited significantly greater EAG responses in 3-day female moths compared to the 1-day female. These compound combinations and the corresponding ratios are probably playing an important role in attracting female adults of C. suppressalis to the vetiver grass.

关 键 词：Chilo suppressalis electroantennography （EAG） VOLATILES vetiver grass trapping mechanism ATTRACTION 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]