Engineering Novel Molecular Beacon Constructs to Study Intracellular RNA Dynamics and Localization  

作　　者：Zhao Ma Xiaotian Wu Christopher J.Krueger Antony K.Chen 

机构地区：[1]Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China [2]Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332 USA

出　　处：《Genomics, Proteomics & Bioinformatics》2017年第5期279-286,共8页基因组蛋白质组与生物信息学报（英文版）

基　　金：supported by grants from the National Key R&D Program of China (Grant Nos. 2016YFA0501603 and 2016YFA0100702);the National Natural Science Foundation of China (Grant Nos. 31771583 and 81371613);the Beijing Natural Science Foundation (Grant No. 7162114);the 1000 Young Talent Program of China

摘　　要：With numerous advancements in novel biochemical techniques, our knowledge of the role of RNAs in the regulation of cellular physiology and pathology has grown significantly over the past several decades. Nevertheless, detailed information regarding RNA processing, trafficking, and localization in living cells has been lacking due to technical limitations in imaging single RNA transcripts in living cells with high spatial and temporal resolution. In this review, we discuss tech- niques that have shown great promise for single RNA imaging, followed by highlights in our recent work in the development of molecular beacons （MBs）, a class of nanoscale oligonucleotide-probes, for detecting individual RNA transcripts in living cells. With further refinement of MB design and development of more sophisticated fluorescence microscopy techniques, we envision that MB-based approaches could promote new discoveries of RNA functions and activities.With numerous advancements in novel biochemical techniques, our knowledge of the role of RNAs in the regulation of cellular physiology and pathology has grown significantly over the past several decades. Nevertheless, detailed information regarding RNA processing, trafficking, and localization in living cells has been lacking due to technical limitations in imaging single RNA transcripts in living cells with high spatial and temporal resolution. In this review, we discuss tech- niques that have shown great promise for single RNA imaging, followed by highlights in our recent work in the development of molecular beacons （MBs）, a class of nanoscale oligonucleotide-probes, for detecting individual RNA transcripts in living cells. With further refinement of MB design and development of more sophisticated fluorescence microscopy techniques, we envision that MB-based approaches could promote new discoveries of RNA functions and activities.

关 键 词：Y-O-methyl RNA PHOSPHOROTHIOATE Molecular beacon RNA dynamics Single-molecule RNAimaging 

分 类 号：Q75[生物学—分子生物学]