黄药子配伍甘草对LO2细胞膜通透性影响的研究  

Study on Effect of Synergy of Glycyrrhiza uralensis and Dioscorea bulbifera on LO2 Cells Membrane Permeability

作　　者：卓实[1] 江川[1] 黄玉芳[1] 潘艳琳[1] 

机构地区：[1]福建中医药大学附属人民医院药学部,福建福州350004

出　　处：《中国现代中药》2017年第10期1397-1399,共3页Modern Chinese Medicine

摘　　要：目的:研究甘草配伍能减轻黄药子对人肝细胞(LO2)膜通透性的影响。方法:激光扫描共聚焦显微镜(LSCM)对经二乙酸荧光素(FDA)染色后的LO2细胞进行实时动态荧光强度检测。通过比较荧光强度的降低率来考察黄药子及其甘草配伍后含药血清在短时间内对细胞膜通透性的影响。结果:LSCM记录细胞荧光强度变化,在10 min扫描停止时,黄药子各组荧光强度降低率与空白组有统计学差异(P<0.05),而甘草配伍组对荧光强度的影响较黄药子组有统计学差异(P<0.05),与空白组无差异(P>0.05)。结论:黄药子含药血清短时间内即影响肝细胞膜通透性,甘草配伍后可明显减弱损伤作用,黄药子引起肝损伤的机制可能是其对肝细胞膜的直接损伤作用,甘草配伍减毒作用与此途径有关。Objective： To study the synergic effect of Glycyrrhiza uralensis and Dioscorea bulbifera on LO2 Cells Membrane Permeability. Methods： The real-time dynamic detection method LSCM was applied to monitor the fluorescence in- tensity of the LO2 cells staining by fluoesceim diacetate with the laser scanning confocal microscope, then the synergic effect of D. bulbifera and G. uralensis on LO2 cells membrane permeability was observed by comparing the reduced rate of fluorescence intensity. Results： When scanning was stopped at the tenth minutes, there was a significant difference between the blank group and D. bulbifera groups in reducing the fluorescence intensity of cells （ P 〈 0. 05 ） , while the G. uralensis groups had no obvious influence on the fluorescence intensity of cells. The changes of fluorescence intensity of cells were recorded by LSCM. Conclusion： D. bulbifera containing serum has an effect on the hepatocyte membrane permeability in a short time after administration, while its compatibility group of G. uralensis and the serum group have no significant effects on it. The hepatic injuries caused by D. bulbifera may be via a coup injury mechanism, but this injury can be weaken by synergy of G. uralensis.

关 键 词：黄药子 甘草 膜通透性 

分 类 号：R135.3[医药卫生—劳动卫生]