20%丁香菌酯悬浮剂高效液相色谱内标分析方法  

Analysis of Coumoxystrobin 20% SC by HPLC and Internal Standard Method

作　　者：许艳秋[1] 高婷婷 王广成[1] 高立明[1] 吴春先[1] 陈炳坤[1] 李婷[1] 唐丽霖 

机构地区：[1]四川省农药检定所,成都610041

出　　处：《农药》2017年第11期812-814,共3页Agrochemicals

摘　　要：[目的]丁香菌酯是一种新型杀菌剂,其分析方法未见文献报道,因此建立了其快捷、高效的分析方法。[方法]采用反相液相色谱内标法,使用ODS C_(18)不锈钢色谱柱,以联苯为内标物,以甲醇-水(体积比75:25)为流动相,以二极管阵列检测器在230 nm处对20%丁香菌酯悬浮剂进行定量分析。[结果]在该色谱条件下基线平稳,丁香菌酯、内标物和杂质能得到较好地分离,保留时间适中。丁香菌酯的平均回收率为100.01%,标准偏差为0.1260,变异系数为0.62%,线性相关系数为0.9998。[结论]该方法简便、快捷,为丁香菌酯质量控制以及质检机构的检测提供了准确、有效的分析方法。[Aims] The analytical method for new fungicide coumoxystrobin has not been reported. [Methods] The RP-HPLC method for the analysis of coumoxystrobin 20% SC with stainless steel column packed with ODS was described. The sample was dissolved with methanol, and the mixture of methanol and water （75：25, by vol） were used as the mobile phase, biphenyl were used as internal standard. Then the target was detected by PDA at 230 rim. [Results] The average recovery of coumoxystrobin was 100.01%, the standard deviation was 0.1260, the variation coefficient was 0.62%, and the linear correlation coefficient was 0.9998. [Conclusions] The method is simple and quick, which provides an accurate and effective analytical method for quality control of coumoxystrobin.

关 键 词：高效液相色谱法 丁香菌酯 内标法 

分 类 号：O657.72[理学—分析化学] TQ455[理学—化学]