骨髓间充质干细胞对苯所致小鼠造血损伤修复作用  

Repair effects of bone marrow mesenchymal stem cells on benzene-induced hematopoietic injury in mice

作　　者：赵娜[1] 宋向荣[1] 刘秋英[1] 李宏玲[1] 蔡学敏[2] 王海兰[1] 

机构地区：[1]广东省职业病防治院,广东省职业病防治重点实验室,广东广州510300 [2]成都军区昆明总医院干细胞与免疫细胞生物技术国家地方联合工程实验室,云南昆明650032

出　　处：《中国职业医学》2017年第5期537-541,共5页China Occupational Medicine

基　　金：国家科技支撑计划项目(2014BAI12B01);国家临床重点专科建设项目(2011-09);广东省职业病防治重点实验室(2012A061400007);广东省科技计划项目(2014A020212551,2014A020212500,2016A020215131);广东省医学科研基金(A2015624,A2016155,A2016458);云南省卫生科技计划项目(2014NS132)

摘　　要：目的探讨骨髓间充质干细胞(BMSCs)对苯中毒小鼠造血损伤的修复作用。方法取无特定病原体级健康雄性昆明小鼠5只,以全骨髓贴壁法分离培养BMSCs。取同类小鼠,以苯油混合物进行多点皮下注射染毒(连续10周,每周3次),建立苯中毒动物模型后,随机分为苯中毒组和BMSCs移植组,每组18只,分别经尾静脉输注250.0μL的0.9%氯化钠溶液或细胞密度为2×10~9/L的BMSCs,每周1次,连续治疗4周。另设对照组小鼠(10只),不予任何处理。治疗结束2周后处死各组小鼠,检测血常规,以瑞氏-吉姆萨法染色观察小鼠骨髓有核细胞,计数骨髓造血祖细胞集落数量,以酶联免疫吸附实验检测小鼠血清中γ-干扰素(IFN-γ)水平。结果成功制备苯中毒动物模型。BMSCs移植疗程结束后,苯中毒组小鼠周围血中白细胞计数、血小板计数、红细胞计数、血红蛋白水平和骨髓中有核细胞总数、粒细胞-巨噬细胞集落形成单位(CFU-GM)均低于对照组(P<0.01),BMSCs治疗组小鼠上述指标均高于苯中毒组(P<0.05);BMSCs治疗组小鼠血小板计数、红细胞计数和骨髓中有核细胞总数、CFU-GM均低于对照组(P<0.05)。苯中毒组小鼠血清中IFN-γ水平高于对照组(P<0.01);BMSCs治疗组小鼠该指标低于苯中毒组(P<0.01),但与对照组比较,差异无统计学意义(P>0.05)。结论 BMSCs对苯所致的造血系统损伤具有修复作用。Objective To explore the repair effects of bone marrow mesenchymal stem cells（ BMSCs） on hematopoietic injury induced by benzene poisoning in mice. Methods Five specific pathogen free healthy male Kunming mice were selected to obtain BMSCs through bone marrow attachment culturing method. The Kunming mice were randomly divided into poisoning group and BMSCs transplantation group,18 mice in each group,after the benzene poisoning model was established by subcutaneous multi-point injection of benzene and oil mixture 3 times/week,10 weeks continuously. Each group was injected through tail vein with 250. 0 μL 0. 9% sodium chloride solution or 250. 0 μL BMSCs suspension（ cell density 2 × 109/L） once per week for 4 weeks,respectively. The control group（ 10 mice） was not given any treatment.Mice were euthanized 2 weeks after treatment. The blood routine examination was conducted. Nucleated cells in bone marrow were observed after Giemsa staining. The clones of hemopoietic progenitor cells were counted and the levels of serum interferon-γ（ IFN-γ） were examined using enzyme-linked immune sorbent assay. Results The mouse model of chronic benzene poisoning was established successfully. After the BMSCs transplantation treatment,the white blood cell count,platelet count,red blood cell count,hemoglobin level and bone marrow nucleated cell as well as granulocyte-macrophage colony forming unit（ CFU-GM） in benzene poisoning group were significantly decreased compared with control group（ P〈0. 01）,while those indexes of BMSCs treatment group were higher than that of benzene poisoning group（ P〈0. 05）. The counts of platelet,red blood cell,bone marrow nucleated cell and CFU-GM in BMSCs treatment group were significantly lower than that of control group（ P〈0. 05）. The level of serum IFN-γ in benzene poisoning group was higher than that of control group（ P〈0. 01）,and serum IFN-γ level in BMSCs treatment group was lower than that of benzene poisoning group（ P〈0. 01）. There was no si

关 键 词：骨髓 间充质干细胞 苯 造血损伤 移植 修复作用 Γ-干扰素 

分 类 号：R135.12[医药卫生—劳动卫生] R114[医药卫生—公共卫生与预防医学]