槲皮素作为饲料添加剂在体内和体外致突变性潜力的评价  

Evaluation of the potential mutagenicity of quercetin as feed additives in vivo and in vitro

作　　者：王盛楠 周博 肖风林 王密 姚佳颖 杨家新 李垚[1] 尹文哲[2] 

机构地区：[1]东北农业大学动物营养研究所,黑龙江哈尔滨150030 [2]哈尔滨医科大学第二附属医院,黑龙江哈尔滨150086

出　　处：《饲料工业》2017年第20期6-10,共5页Feed Industry

基　　金：黑龙江省自然科学基金项目[C2016017];哈尔滨市科技局基金项目[2015RQXXJ014]

摘　　要：研究通过致突变性检测(Ames)试验、骨髓微核试验以及精子畸形试验,以进一步确定槲皮素作为饲料添加剂在体内和体外致突变性的潜力。体内骨髓微核试验采用昆明种小鼠50只(雌、雄各半),随机分5组,每组10个重复。精子畸形试验采用昆明种雄性小鼠50只,随机分5组,每组10个重复;体外试验采用鼠伤寒沙门氏菌突变型菌株(TA97、TA98、TA100和TA102)进行。在肝微粒体多氯联苯诱导剂(9 000×g Supernatant,S9)干预和不干预的情况下,槲皮素各剂量组对鼠伤寒沙门氏菌(TA97、TA98、TA100和TA102)的回复突变菌落数与阴性对照组无显著差异(P>0.05);同时,槲皮素各剂量组雌雄小鼠的骨髓微核率和嗜多染红细胞/红细胞(Polychromatic erythrocyte/Red blood cell,PCE/RBC)值与阴性对照组无显著差异(P>0.05);槲皮素各剂量组雄性小鼠精子畸形率与阴性组无显著差异(P>0.05)。结果表明,在本试验条件下,槲皮素无致突变性和细胞毒性,并且进一步证明槲皮素是一种安全的饲料添加剂。In order to further define the potential in vivo and in vitro mutagenicity of quercetin as feed additives, an Ames assay, a bone marrow micronucleus assay, and a sperm malformation assay were conducted. In vivo, 50 Kunming mice （half male and half female） were randomly divided into 5 groups, and l0 replicates in each group for bone marrow micronucleus assay. 50 Kunming mice （male） were randomly divided into 5 groups, and 10 replicates in each group for sperm malformation assay; in vitro, Salmonella typhimurium mutant strains （TA97, TA98, TA100 and TA102） were tested. Quercetin at dose level of up to 5 mg per vessel did not increase mutant colonies with rat liver microsomal enzyme mixture 9 000Xg supernatant （$9） and without $9 in TA97, TA98, TA100 and TA102. In addition, oral- ly administered quercetin [500, 5 000 mg/（kg. BW） and 10 000 mg/（kg. BW）] did not increase micronu- clear rates and polychromatic erythrocytes （PCE）/red blood cell （RBC） in male and female mice, and quercetin [500, 5 000 mg/（kg-BW） and 10 000 mg/（kg. BW）] did not increase rate of teratosperm in male mice. These results demonstrated that quercetin was not mutagenic and cytotoxic under the condi- tions of this experiment and further proved that quercetin was a safe feed additive in vivo and in vitro.

关 键 词：槲皮素 致突变性 回复突变菌落 骨髓微核 精子畸形 PCE/RBC 

分 类 号：S816.7[农业科学—饲料科学]