米力农对LPS诱导下人冠状动脉内皮细胞炎症反应的影响  

Effects of milrinone on LPS-induced inflammation of human coronary artery endothelial cells

作　　者：李艳珍 和荣丽[2] 陆利[2] 

机构地区：[1]山西医科大学汾阳学院临床医学系,汾阳032200 [2]山西医科大学,太原030000

出　　处：《中国免疫学杂志》2017年第11期1709-1712,共4页Chinese Journal of Immunology

基　　金：山西省高等学校科技项目(No.21021005)

摘　　要：目的:观察米力农对LPS诱导下人冠状动脉内皮细胞(HCAEC)炎症反应及NF-κB的影响。方法:体外培养HCAEC,分为正常组、LPS组(1μg/ml)、米力农50μmol/L组和米力农200μmol/L组,后两组均与LPS共同孵育24 h。分别采用qRT-PCR和ELISA检测IL-6、IL-8、TNF-α的mRNA和蛋白水平,分别采用Western blot和免疫荧光法检测NF-κB活化和核移位。结果:与LPS组相比,米力农显著降低HCAEC的IL-6、IL-8、TNF-α的mRNA和蛋白表达;抑制NF-κB活化和核移位(P<0.05),且呈一定量效关系。结论:米力农可通过抑制NF-κB活化而降低LPS诱导的HCAEC释放炎症因子。Objective：To observe the effects of milrinone on LPS-induced inflammation and NF-κB activation of human coronary artery endothelial cells（HCAEC）.Methods：HCAEC were cultured in vitro,and were divided into normal group,LPS group（1μg/ml）,milrinone 50 μmol/L group and milrinone 200 μmol/L group,the rear two groups were co-incubated with LPS for 24 h.qRTPCR and ELISA was applied to detect IL-6,IL-8,TNF-α mRNA and protein,respectively.Western blot and immunofluorescence assay was used to observe the activation and translocation of NF-κB,respectively.Results：Compared with LPS group,the IL-6,IL-8,TNF-αmRNA and protein;the activation and translocation of NF-κB significantly inhibited after incubation with milrinone（P0.05） in a dose dependent manner.Conclusion：Milrinone inhibited the release of inflammative cytokines induced by LPS of HCAEC in vitro,partly by inactivation of NF-κB.

关 键 词：米力农 HCAEC 炎症 NF-ΚB 

分 类 号：R453.9[医药卫生—治疗学]