低水平HBsAg患者肝组织HBsAg、HBcAg表达与血清HBsAg水平、HBVDNA载量的相关性  

Correlations betweenintrahepatic HBsAg and HBcAg levels and serum HBsAg and HBV DNA levels in low-level HBsAg patients

作　　者：刘立 刘春云 王霖 刘云华 

机构地区：[1]云南省昆明市第三人民医院肝三科,650041 [2]云南省昆明市第三人民医院病理科,650041

出　　处：《实用医学杂志》2017年第21期3639-3642,共4页The Journal of Practical Medicine

基　　金：云南省科技厅应用基础研究自筹经费项目(编号:2010ZG196)

摘　　要：目的分析低水平HBsAg患者肝组织HBsAg、HBc Ag表达与血清HBsAg水平、HBV DNA载量的相关性,为低水平HBsAg患者的病情判断提供依据。方法选取2013年1月至2015年12月HBsAg水平低于1 400 IU/m L且高于0.05 IU/m L者作为研究对象,收集的63例低水平HBsAg患者均进行了肝组织活检。采用罗氏cobas e601全自动电化学发光免疫分析系统及配套试剂检测血清HBsAg,HBV DNA采用ROCHE公司全自动Ampli Prep/cobas Taq Man 48系统检测仪及配套试剂进行检测。结果 63例患者中,肝组织HBsAg表达阳性例数:阴性5例(7.94%)、+有47例(74.60%)、++有8例(12.70%)、+++有2例(3.17%)、++++有1例;而其HBc Ag表达阳性例数:阴性30例(47.62%)、+有28例(44.44%)、++有4例(6.35%)、+++有1例(1.59)、++++有0例。低水平HBsAg患者肝组织HBsAg表达与血清HBsAg水平通过spearman和kendall相关分析均呈显著正相关(spearman分析:r=0.261,P=0.039;kendall分析:r=0.217,P=0.036),而其与血清HBV DNA载量没有显著相关性;肝组织HBc Ag表达与血清HBsAg水平无显著相关性,而与血清HBV DNA载量通过spearman和kendall相关分析均有显著正相关性(spearman分析:r=0.305,P=0.015;kendall分析:r=0.259,P=0.017)。结论低水平HBsAg患者肝组织HBsAg表达与血清HBsAg水平呈显著正相关,而与HBV DNA载量无关;肝组织HBc Ag表达与血清HBV DNA载量有显著正相关性,而与血清HBsAg水平无关。Objective To analyze the relationship between intrahepatic expression of HBsAg and HBc Agand the serum HBsAg and the load of HBV DNA in low-level HBsAg. To provide the basis for the diagnosis of low-level HBsAg. Methods A total of 63 patients with HBsAg lower than 1 400 IU/m L and higher than 0.05 IU/m L wereenrolled in the study. All the 63 patients with low level HBsAg were collected for liver biopsy from January 2013 toDecember 2015. Serum HBsAg was detected by Roche cobas e601 automatic electrochemiluminescence immunoas-say system and supporting reagents. HBV DNA was detected using ROCHE automatic Ampli Prep/cobas Taq Man 48 system detector and supporting reagents. Results Among the 63 patients,the HBsAg in liver tissue was negative in5 cases(7.94%),+ in 47 cases(74.60%),++ in 8 cases(12.70%),+++ in 2 cases(3.17%),and ++++ wasfound in 1 case. The expression of HBc Ag were negative in 30 cases(47.62%),+ in 28 cases(44.44%),++ in4 cases(6.35%),+++ in 1 case(1.59%),and ++++ in 0 cases. There was a significant positive correlation betweenHBsAg expression and serum HBsAg level by spearman and kendall correlation analysis(spearman analysis:r = 0.261,P = 0.039;kendall analysis:r = 0.217,P = 0.036). HBc Ag expression was significantly correlated withserum HBV DNA load with spearman and kendall correlation analysis(spearman analysis:r = 0.305,P = 0.015;kendall analysis:r = 0.259,P = 0.017). Conclusions The expression of HBsAg in liver tissue of in the patientswith low level of HBsAg is positively correlated with the level of serum HBsAg,but not with the load of HBV DNA.There is significant positive correlation between HBc Ag expression and serum HBV DNA load.

关 键 词：低水平HBsAg 肝组织病理 HBCAG 

分 类 号：R512.62[医药卫生—内科学]