BSA-ISCOMs对欧洲鳗鲡免疫效应及抗细菌性病原的效果研究  

Effects of BSA-ISCOMs on the immune responses and the resistance to bacterial pathogens of European eel(Anguilla anguilla)

作　　者：陈秀霞[1] 龚晖[1] 许斌福[1] 郑在予[1] 池洪树[1] 陈永聪[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003

出　　处：《应用海洋学学报》2017年第4期558-564,共7页Journal of Applied Oceanography

基　　金：福建省自然科学基金资助项目(2015J01106);福建省农业科学院科技创新团队重点科研资助项目(CXTD2011-06)

摘　　要：为证实免疫刺激复合物(ISCOMs)的非特异免疫增强作用,将牛血清白蛋白(BSA)制备成ISCOMs(BSA-ISCOMs).将欧洲鳗鲡(Anguilla anguilla)随机分为3组,分别用BSA-ISCOMs、BSA和磷酸盐缓冲液(PBS,对照组)腹腔注射免疫.二次免疫完成后第30d采血,测血清部分非特异性免疫指标,剩余鳗鲡分别用创伤弧菌(Vibrio vulnificus)和嗜水气单胞菌(Aeromona hydrophila)攻毒.结果显示,BSA-ISCOMs组的血清溶菌酶活力显著高于BSA组和对照组(p≤0.05);超氧化物歧化酶(SOD)和补体C3活力与对照组无显著差异(p>0.05).攻毒结果显示,BSA-ISCOMs组的存活率显著高于BSA组和对照组(p≤0.05),对创伤弧菌和嗜水气单胞菌攻击的相对免疫保护率分别为43.3%和51.7%.表明BSA-ISCOMs能有效增强欧洲鳗鲡对主要病原创伤弧菌和嗜水气单胞菌的抗病能力.To test the effects of immune-stimulating complexes( BSA-ISCOMs),which was prepared by bovine serum albumin( BSA),on the non-specific immune responses of Anguillidae fish,European eels( Anguilla anguilla)were immunized intraperitoneally with BSA-ISCOMs or BSA,while the control group were injected with phosphate buffered solution( PBS). On the 30 th day after the second immunization,serum samples were collected for nonspecific immune parameters analysis,and the remaining eels were infected with Vibrio vulnificus and Aeromona hydrophila. The results showed that the eels immunized with BSA-ISCOMs presented significantly higher lysozyme activity than the eels injected with BSA or PBS( p≤0. 05). Superoxide dismutase( SOD) and complement component 3( C3) activities of the BSA-ISCOMs group were not significantly different from those of the BSA or PBS group( p > 0. 05). Survival rates of the BSA-ISCOMs group were significantly higher than those of BSA or PBS group( p≤0. 05),with a relative percent survival( RPS) of 43. 3% for V. vulnificus challenge and 51. 7% for A. hydrophila challenge. This study revealed the potential benefit of BSA-ISCOMs for the immune responses and disease resistance of European eel.

关 键 词：海洋生物学 免疫刺激复合物 欧洲鳗鲡 免疫效应 细菌性病原 溶菌酶 

分 类 号：P735[天文地球—海洋生物学]