丙泊酚对大鼠初级躯体感觉皮层神经元电压门控钾通道的影响  

Effects of propofol on potassium channels of S1 neurons in rats

作　　者：何炯策 张宇[2] 刘兴奎[2] 喻田[2] 黄千瑜 HE Jiongce;ZHANG Yu;LIU Xingkui;YU Tian;HUANG Qianyu(Department of Anesthesia and Perioperative Medicine, the Second Affliated Hospital of Wenzhou Medical University, Wenzhou, 325027;Department of Anaesthesiology, Zunyi Medical College, Zunyi, 563003)

机构地区：[1]温州医科大学附属第二医院麻醉与围术期医学科,浙江温州325027 [2]遵义医学院麻醉学系,贵州遵义563003

出　　处：《温州医科大学学报》2017年第10期713-717,共5页Journal of Wenzhou Medical University

基　　金：国家自然科学基金资助项目(81060266)

摘　　要：目的:探讨丙泊酚对大鼠S1区神经元电压门控钾通道的影响。方法:使用全细胞记录脑片膜片钳技术测定神经元细胞外向钾电流。人工脑脊液中灌流不同浓度(10、30、100、300μmol/L)丙泊酚,记录各组瞬间外向钾电流(IA)和延迟整流钾电流(IK),绘制外向钾通道电流电压(IV)曲线、稳态激活曲线并计算相关参数。结果:丙泊酚呈浓度依赖性抑制大鼠S1区神经元电压门控性IA,延缓瞬间外向钾通道的激活,使稳态激活曲线向去极化方向移动。同时丙泊酚呈浓度依赖性抑制大鼠S1区神经元电压门控性IK,但不影响延迟整流钾通道的激活。结论:丙泊酚对大鼠丘脑皮层环路中的S1区神经元电压门控钾通道的抑制作用可能是其诱导的全麻作用的机制之一。Objective： To explore the effects of propofol on voltage-gated potassium channels of primary S1 neurons in rats. Methods： Whole-cell patch clamp technique was used to record potassium currents of neurons. After application with propofol at different concentrations（10-300 μmol/L） in ACSF, the currents of outward potassium currents（IA） and delayed rectifier potassium currents（IK） were recorded to make current-voltage curve, steady-state activation curve and account characteristic parameters. Results： Propofol inhibited IA of S1 neurons in rats in a dose-dependent manner. It also inhibited the activation of IA, shifted the activation curve towards the deperpolarzating potential. Propofol inhibited IK of S1 neurons of rats in a dose-dependent manner. But it did not affect activation of IK. Conclusion： The results of this study provide new vidence that propofol shows an inhibitory effect on voltage-gated potassium channels in the thalamocortical circuit, which may play a role in the mechanisms of propofol-induced general anesthesia.

关 键 词：丙泊酚 大鼠 膜片钳 钾通道 

分 类 号：R614.1[医药卫生—麻醉学]