红景天苷对缺氧缺糖诱导的星形胶质细胞线粒体自噬的影响  

The effect of salidroside on mitophagy induced by oxygen-glucose deprivation in astrocytes

作　　者：郑丽云[1] 黄慧芬[1] 邱伟文 ZHENG Liyun;HUANG Huifen;QIU Weiwen(Department of Neurology, the Fifth Affliated Hospital of Wenzhou Medical University, Lishui, 323000;Department of Neurology, the Sixth Affliated Hospital of Wenzhou Medical Univer-sity, Lishui, 323000)

机构地区：[1]温州医科大学附属第五医院神经内科,浙江丽水323000 [2]温州医科大学附属第六医院神经内科,浙江丽水323000

出　　处：《温州医科大学学报》2017年第10期744-747,共4页Journal of Wenzhou Medical University

摘　　要：目的:探讨红景天苷(Sal)对缺氧缺糖(OGD)诱导的星形胶质细胞线粒体自噬的影响。方法:提取原代培养的新生鼠皮层星形胶质细胞,进行OGD干预,建立OGD细胞模型。将实验分为对照组、OGD组和Sal+OGD组。用噻唑蓝(MTT)法检测细胞存活率,乳酸脱氢酶(LDH)法检测LDH漏出率评估细胞损伤,Westernblot法检测自噬相关蛋白表达水平。结果:Sal终浓度为75、150、300μmol/m L时,细胞存活率分别为83.15%±5.42%、86.75%±4.91%、91.41%±5.38%,明显高于OGD组(71.22%±8.13%),但仍低于对照组(99.39%±2.17%),差异均有统计学意义(P<0.05);LDH漏出率为34.61%±5.19%、30.51%±8.15%、27.34±7.41%,明显低于OGD组(40.51%±8.15%),但仍高于对照组(16.68%±3.69%),差异均有统计学意义(P<0.05);Westernblot结果显示Sal+OGD组中LC3、Beclin1、PINK1和Parkin蛋白表达较OGD组明显降低,但仍高于对照组(P<0.05)。结论:Sal抑制OGD诱导线粒体自噬相关蛋白表达,降低OGD诱导的星形胶质细胞损伤。Objective： To investigate the effect of salidroside（Sal） on mitophagy induced by oxygen-glucose deprivation（OGD） in astrocytes. Methods： The primary astrocytes were isolated from cerebral cortex of neonatal rats and subjected to OGD for imitating hypoxia and glucose deprivation model in vitro. Cells were divided into sham group, OGD model group and Sal pretreatment＋OGD group（Sal＋OGD）. Cell survival rate was detected with MTT and LDH leakage was analyzed with lactate dehydrogenase activity assay kit. Western blot was used to detect the expression of autophagy related protein. Results： The survival rate of cells with final concentration of 75, 150, 300 mol/m L Sal pretreatment and OGD were 83.15%±5.42%, 86.75%±4.91%, 91.41%±5.38%, which were significantly higher than that of the model group（71.22%±8.13%）（P0.05）, but still lower than that of the control group（99.39%±2.17%）, the difference was statistically significant（P0.05）. Meanwhile, the LDH leakage rate of cell with final concentration of 75, 150, 300 mol/m L Sal pretreatment were 34.61%±5.19%, 30.51%±8.15%, 27.34%±7.41%, which was significantly lower than that of model group（40.51%±8.15%）, but still higher than that of the control group（16.68%±3.69%）, the difference was statistically significant（P 0.05）. Western blot showed the expression of LC3, Beclin1, PINK1 and Parkin protein in the Sal＋OGD group significantly lower than that in model group, but still higher than that in the control group（P0.05）. Conclusion：Sal decreases mitophagy related protein and restored the cell proliferation inhibition induced by OGD in astrocytes.

关 键 词：红景天苷 线粒体自噬 缺氧缺糖 星形胶质细胞 细胞损伤 

分 类 号：R285[医药卫生—中药学] R743.1[医药卫生—中医学]