大黄素对实验性自身免疫性甲状腺炎模型小鼠T细胞亚群和IFN-γ、IL-4的影响  

Effects of Emodin on T Cell Subsets and IFN-γ, IL-4 in Autoimmune Thyroiditis Mice

作　　者：吴可人[1] 叶志鹏[1] 黄捷 金法[1] 方蓉[1] 徐涛[1] 李宁[1] 

机构地区：[1]浙江中医药大学第一附属医院肝胆外科,杭州310006

出　　处：《浙江中西医结合杂志》2017年第11期923-926,910,1021,共6页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省中医药科技计划项目(No.2014ZA037)

摘　　要：目的大黄素对实验性自身免疫性甲状腺炎模型小鼠T细胞亚群和IFN-γ、IL-4的影响。方法通过过量碘剂诱导NOD小鼠建立EAT动物模型,造模4周后各实验组摄入不同剂量的大黄素。造模8周后检测小鼠血浆TgAb水平及甲状腺炎症程度。流式细胞术检测小鼠外周血、脾脏T细胞亚群,分析大黄素对EAT小鼠外周血、脾脏淋巴细胞IFN-γ和IL-4分泌能力的影响。结果(1)甲状腺病理学观察,大黄素实验组较模型组甲状腺淋巴细胞浸润程度减轻;分级比较有显著性差异(P<0.01);(2)造模后模型组小鼠血浆Tg Ab水平较对照组明显升高[(44.16±3.59)ng/mL比(11.24±1.52)ng/mL],大黄素实验组升高幅度低于模型组[(26.58±5.24)ng/mL、(19.18±5.73)ng/mL、(23.24±5.47)ng/mL比(44.16±3.59)ng/mL],各组间有统计学差异(P<0.01);(3)大黄素实验组外周血单核细胞、脾脏淋巴细胞中CD3+CD4+、CD3+CD8+T淋巴细胞的频率较对照组明显升高,外周血单核细胞[(7.72±2.92)%比(4.13±1.45)%,(4.23±1.58)%比(2.76±1.69)%];脾脏淋巴细胞[(7.51±1.31)%比(1.82±1.35)%,(5.59±1.98)%比(0.033±0.034)%],但低于模型组外周血单核细胞[(7.72±2.92)比(17.46±3.71)%,(4.23±1.58)%比(8.92±2.62)%;脾脏淋巴细胞[(7.51±1.31)%比(16.74±4.79)%,(5.59±1.98)%比(11.97±2.21)%](P<0.01);而CD3+CD4+FIN-γ+、CD3+CD4+IL-4+T细胞频率高于对照组但明显低于模型组(P<0.01)。结论过量碘剂诱导NOD小鼠自身免疫性甲状腺炎造模是可行的;大黄素对造模小鼠甲状腺炎有一定保护作用。大黄素通过抑制CD4+、CD8+T淋巴细胞分化并且抑制IFN-γ和IL-4的分泌,从而抑制造模小鼠的自身免疫反应。Objective To investigate the protective effect of emodin on T cell subsets and the secretion of IFN-γ and IL-4 in autoimmune thyroiditis( EAT) mice. Methods Four weeks after modeling, experimental groups were treated with emodin with different doses. The TgAb level in plasma and thyroid inflammation were detected 8 weeks after modeling to evaluate the protective effect of emodin on EAT mice. T cell subset and the levels of IFN-γ and IL-4 in peripheral blood and spleen were detected by flow cytometry. Results The histopathological study revealed that inflammatory infiltration in thyroid was significant reduced after emodin treatment compared with control group( P<0.01). Levels of serum TgAb were significant increased in each group after modeling[( 44.16±3.59)ng/mL vs( 11.24±1.52) ng/mL], and the increasing amplitudes in emodin treated groups were significantly less than that in model group[( 26.58±5.24) ng/mL,( 19.18±5.73) ng/mL,( 23.24±5.47) ng/mL vs( 44.16±3.59) ng/mL, P<0.01]. After modeling, the cell frequencies of CD3+CD4+, CD3+CD8+T cells, CD3+CD4+IL-4+ and CD3+CD4+IFN-γ+ T cells in peripheral blood monocyte and splenic lymphocyte were significant increased in each group compared with control group peripheral blood monocyte:[( 7.72%±2.92) % vs( 4.13%±1.45) %,( 4.23%±1.58) % vs( 2.76%±1.69) %];splenic lymphocyte:[( 7.51%±1.31) % vs( 1.82%±1.35) %,( 5.59%±1.98) % vs( 0.033±0.034) %,P<0.01], while the increasing amplitudes in emodin treated groups were less than that in model group peripheral blood monocyte:[( 7.72±2.92) % vs( 17.46±3.71) %,( 4.23±1.58) % vs( 8.92%±2.62) %]; splenic lymphocyte:[( 7.51±1.31) % vs( 16.74±4.79) %,( 5.59±1.98) % vs( 11.97±2.21) %, P<0.01]. The difference in cell frequencies of CD3+CD4+IFN-γ+ and CD3+CD4+IL-4+T cells in emodin treated groups were significant higher than that of control group and lower than that of model group( P<0.01). Conclusion The EAT model is visible through an excessive iodine-induced method in NOD mice, and emodin shows a certain inhi

关 键 词：NOD小鼠 实验性自身免疫性甲状腺炎 大黄素 T细胞亚群 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]