髓系抑制性细胞和调节性T细胞在小鼠实验性结肠炎中的作用  

Role of myeloid-derived suppressor cells and regulatory T cells in mouse models of ulcerative colitis

作　　者：吕颖[1] 卢建华[2] 吕卓 段媛媛[3] 刘志权[2] 崔美兰[1] 闫会敏[1] 

机构地区：[1]河北省石家庄市第五医院临床医学研究中心,河北石家庄050021 [2]河北省石家庄市第五医院检验科,河北石家庄050021 [3]河北医科大学研究生学院,河北石家庄050017

出　　处：《中国现代医学杂志》2017年第27期17-21,共5页China Journal of Modern Medicine

基　　金：河北省自然科学基金(No:H2015106081);河北省医学科学研究课题计划项目(No:20150893)

摘　　要：目的探讨髓系抑制性细胞(MDSC)和调节性T细胞(Treg细胞)在小鼠溃疡性结肠炎发生、发展过程中的作用。方法使用5%右旋葡聚糖硫酸钠(DSS)溶液复制小鼠溃疡性结肠炎模型,每日观察小鼠体重和大便状况。DSS处理3和7 d后处死小鼠,苏木精-伊红染色法观察病理组织学变化,流式细胞仪检测脾脏和肠系膜淋巴结中MDSC和Treg细胞的表达。结果模型组小鼠于造模第4天体重开始下降,有腹泻和血便,HE染色结果显示小鼠结肠出现黏膜损伤及炎症表现。与对照组和造模3 d组比较,造模7 d组肠系膜淋巴结中Treg细胞比例增加,而MDSC比例降低;进一步观察MDSC亚群变化发现,粒细胞型MDSC在造模7 d组中比例下降,而单核细胞型MDSC则无改变。无论Treg细胞还是MDSC,脾脏中的水平3组比较差异无统计学意义。结论急性溃疡性结肠炎可导致MDSC降低和Treg细胞增高,可能与疾病的发生发展有关。Objective To investigate the role of myeloid-derived suppressor cells （MDSC） and regulatoryT（Treg） cells in development of ulcerative colitis. Methods Mouse models of ulcerative colitis were establishedwith administration of5豫 dextran sodium sulfate （DSS）. Body weight and hematochezia were obtained daily.Mice were sacrificed3 or 7 days post insult. Histological assessment of ulcerative colitis was graded withhematoxylin -eosin staining. The percentages of MDSC and Treg cells in the spleen and mesenteric lymphnodes were measured by flow cytometry. Results In animals treated with DSS, body weight loss, diarrhea andhematochezia were observed on the 4th day. Histological analysis revealed colonic mucosa damage andmanifestation of inflammation. The amount of Treg cells was increased, whereas the amount of MDSC wasdecreased significantly in mesenteric lymph nodes in 7-day model group（ 〈 0.05）. Further observation on thesubsets of MDSC suggested that the percentage of granulocyte -like MDSC decreased in the 7 -day modelgroup （ 〈 0.05） while no significant change of monocyte-like MDSC was observed among all the groups.There was no significant difference in the amount of Treg cells or MDSC in spleen among three groups.Conclusion Acute ulcerative colitis experiences decreased MDSC and increased Treg cells, which couldcontribute to the development of ulcerative colitis.

关 键 词：溃疡性结肠炎 髓系抑制性细胞 调节性T细胞 免疫调控 

分 类 号：R392.9[医药卫生—免疫学]