基于分子印迹聚合物膜的胰蛋白酶电化学传感器研究  

Research of electrochemical sensor for trypsin based on molecular imprinted polymer membrane

作　　者：郑黎云 李艳霞[1] 

机构地区：[1]闽江学院化学与化学工程系,福建福州350108

出　　处：《闽江学院学报》2017年第5期73-79,共7页Journal of Minjiang University

基　　金：国家自然科学基金资助项目(21405075);福建省自然科学基金(2016J05040);福建省高校杰出青年人才培育计划;福建省高校样报世纪优秀人才支持计划

摘　　要：构建了一种高灵敏度、高选择性的胰蛋白酶(Try)分子印迹电化学传感器(MIECS).首先,采用电沉积法将氯金酸溶液沉积在GCE表面,得到Au NPs修饰的玻碳电极(GCE).接着,通过自由基引发诱导使丙烯酰胺在模板蛋白共存的溶液中氧化还原聚合反应形成分子印迹聚合物层,模板蛋白经洗脱液的洗脱后形成MIECS.同时对模板蛋白洗脱液的选择、洗脱时间、孵化时间进行了优化.结果表明,所构建的MIECS在优化条件下,胰蛋白酶浓度在0.5~1.0×103μg·m L-1范围内MIECS表现出良好的电化学响应,可用于Try的识别与分析.A kind of high sensitivity and selectivity of molecular imprinted electrochemical sensor for trypsin was constructed. First,we use electrodeposition to deposit chloroauric acid in solution on the surface of GCE modified by Au NPs. Then the molecularly imprinted polymer layer was formed by redox polymerization in the solution of template protein coexistence induced by free radical. After elution of template protein,a kind of MIECS was successfully formed. Furthermore,the choice of template protein eluent,elution time and incubation time were optimized. The results show that the constructed MIECS under the optimized conditions have a good electrochemical response,with concentration of trypsin in the range of 0.5 ~ 1.0×103μg·m L-1,which can be used to the identify and analyze trypsin.

关 键 词：分子印迹技术 电化学传感器 胰蛋白酶 纳米金 

分 类 号：O657.32[理学—分析化学]