骨松强骨方对去卵巢大鼠股骨骨密度、骨强度和骨结构的影响  

Effects of Gusongqianggu Decoction on Bone Mineral Density,Bone Strength and Bone Structure of Ovariectomized Rats

作　　者：朱静栋 孔西建 黄付伟 李盈盈 吴丹 毛春焕 叶进 王咪娜 王璘琳 

机构地区：[1]安徽芜湖市中医医院中医住院医师规范化培训基地,安徽芜湖241000 [2]河南省洛阳正骨医院(河南省骨科医院)骨质疏松症与代谢性骨病研究中心,河南省洛阳471002 [3]湖南中医药大学,湖南长沙410000

出　　处：《中国中医骨伤科杂志》2017年第11期1-6,共6页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：国家中医临床研究基地业务建设科研专项(JDZX2015037)

摘　　要：目的:研究骨松强骨方对去卵巢大鼠股骨骨密度、骨强度和骨结构的影响。方法:采用去势雌性大鼠作为骨质疏松动物模型,将其分为假手术组,对照组,雌激素组,骨松强骨方低、中、高治疗组。治疗3个月后,检测大鼠骨密度、股骨松质骨显微结构以及股骨的生物力学性能。结果:对照组与假手术组相比骨密度、骨应力、结构强度、弹性模量、骨小梁数目显著降低,骨小梁间隙、骨小梁模型因子显著增高,表明摘去卵巢后成功建立骨质疏松症的病理模型。各治疗组与对照组进行比较,发现雌二醇和骨松强骨方的中剂量、高剂量组均能显著抑制大鼠骨密度降低;雌激素及骨松强骨方可不同程度地提高大鼠骨应力、弹性模量,改善大鼠股骨干骺端骨小梁的显微结构。结论:骨松强骨方对骨质疏松症的治疗具有较好的防治作用,且呈剂量依赖性。通过抑制骨量流失,改善骨小梁微结构,提高股骨强度,从而预防脆性骨折。Objective：To study the effect of Gusongqianggu decoction（GQGD）on the bone mineral density,bone strength,and bone structure of ovariectomized rats.Methods：Using ovariectomized rats established osteoporosis animal model.The rats were divided into sham operation group,control group,estrogen group,and low,middle and high-doses of GQGD treatment groups.After three months of treatment,the bone mineral density,cancellous bone microstructure,and biomechanical properties of the femur were measured.Results：Compared with the sham group,the bone density,bone stress,structural hardness,elastic modulus,and the number of trabecular bone of the control group were significantly reduced,while the trabecular space and the trabecular model factor were significantly increased.It was indicated that the pathological model of osteoporosis was established successfully.The treatment groups were compared with the control group,results showed that estradiol and GQGD in the middle-dose and high-dose group can significantly inhibit the loss of bone mineral density.Estrogen and GQGD could increase the rat bone stress and elastic modulus,improve the microstructure of trabecular bone in metaphysis of femur in rats.Conclusion：The experimental result shows that treating osteoporosis by GQGD is effective and at dose-dependent,which by inhibiting the loss of bone mass,improving the microstructure of bone trabecular,and increasing the strength of the femur to prevent the risk of brittle fracture.

关 键 词：骨松强骨方 去卵巢雌性大鼠 骨密度 骨显微结构 骨强度 

分 类 号：R33[医药卫生—人体生理学]