甘肃汉族人群COL9A2基因多态性与腰椎间盘突出症术后复发的相关性研究  

Correlation Research of COL9A2 Gene Polymorphism in Gansu Han Population and Postoperative Recurrence of Lumbar Disc Herniation

作　　者：王兴盛[1] 王想福[2] 赵宁[2] 范有福[2] 孙凤岐[2] 

机构地区：[1]甘肃省中医药研究院,兰州730050 [2]甘肃省中医院,兰州730050

出　　处：《中国中医骨伤科杂志》2017年第11期7-10,共4页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：甘肃省自然科学基金资助项目(1310RJZA053)

摘　　要：目的:探讨COL9A2基因rs12077871和rs3737820位点基因多态性与甘肃汉族人群腰椎间盘突出症术后复发的关系。方法:分析来自2006年3月至2013年3月甘肃省中医院脊柱骨科就诊并行手术治疗,且年龄、性别相匹配的腰椎间盘突出症患者行髓核摘除术后无复发且无临床症状者(95例)为对照组;髓核摘除术后复发且有临床症状者(82例)为病例组。收集两组患者外周血样本,提取DNA,应用聚合酶链反应-连接酶检测反应(PCR-LDR)法分析病例组与对照组rs12077871和rs3737820位点基因型及等位基因频率,采用"病例-对照"方法分析基因型相关性。结果:rs12077871位点的各基因型与等位基因频率分布在病例组和对照组之间差异有统计学意义(P<0.05)。而rs3737820位点的各基因型与等位基因频率分布在病例组和对照组之间均差异无统计学意义(P>0.05)。结论:COL9A2rs12077871位点基因多态性与甘肃汉族人群腰椎间盘突出症术后复发易感性存在关联性。Objective：To explore the relationship between the gene polymorphism of rs12077871 and rs3737820 locus in COL9 A2 and the postoperative recurrence of lumbar disc herniation in Han population in Gansu province.Methods：An analysis was performed on the patients with lumbar disc herniation who went to the department of orthopedics in Gansu provincial hospital of traditional Chinese medicine（TCM）and was treated with operation from March 2006 to March 2013.In addition,their age and gender were matched.The patients who had no recurrence and no clinical symptoms after the micro endoscopic discectomy（MED,95 cases）were divided into the normal group.And the other who had recurrence and clinical symptoms after MED（82 cases）were divided into the case group.First,the blood sample of the two groups were collected.Secondly,the DNA was extracted from the sample.Then the genotype and allele frequency of rs12077871 and rs3737820 between the normal and case group were analyzed by PCR-LDR.Finally,the correlation of genotype was analyzed according to the ways of the case-control study.Results：The genotype and allele frequency at rs12077871 locus had significant difference between the two group（P〈0.05）.However,there was no significant difference at rs3737820 locus between the two group（P〉0.05）.Conclusion：There is an association between the gene polymorphism of rs12077871 locus in COL9 A2 and the susceptibility of postoperative recurrence of lumbar disc herniation in Han population in Gansu Province.

关 键 词：腰椎间盘突出症术后 COL9A2 基因多态性 

分 类 号：R681.5[医药卫生—骨科学]