体外诱导树鼩骨髓间充质干细胞向神经元样细胞分化的实验研究  

Induction and Differentiation of Tree Shrew Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells in Vitro

作　　者：苗雨润[1] 李娜[1] 匡德宣[1] 宋庆凯 袁圆 王璇 张志成 陆彩霞[1] 

机构地区：[1]中国医学科学院/北京协和医学院医学生物学研究所云南省重大传染病疫苗研发重点实验室树鼩种子资源中心,昆明650118

出　　处：《实验动物与比较医学》2017年第5期337-343,共7页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金项目(No.31601907);实验树鼩标准化与应用研究云南省创新团队;云南省联合支持国家计划项目(No.2015GA009)

摘　　要：目的探讨树鼩骨髓间充质干细胞(BM-MSCs)向神经元样细胞分化的可行性。方法通过采用不同诱导物浓度的神经元样细胞诱导液对体外培养的树鼩BM-MSCs向神经元样细胞进行诱导分化,用PCR法检测细胞神经元烯醇化酶(NES)和α-突触核蛋白(α-SYN)表达情况,并用NES抗体进行细胞免疫荧光染色鉴定。结果使用浓度为10 ng/mL表皮生长因子(EGF)和20 ng/mL碱性成纤维细胞生长因子(bFGF)诱导液在体外诱导培养树鼩BM-MSCs至12 d时可以得到良好诱导结果。结论优化的双细胞因子诱导培养液可以成功将树鼩BM-MSCs诱导分化为神经元样细胞,分化的细胞符合神经元样细胞的一般特征。Objective To observe the feasibility of the differentiation of tree shrew （Tupaia belangeri） bone marrow mesenchymal stem cells （BM-MSCs） to neuron-like cells. Method The tree-shrew BM-MSCs were differentiated to neuron-like cells by IMEM differentiation culture mediums （with different inducer and concentration）. Identify the secretion concentration of neuron-specific enolase （NES） and α-synuclein （α-SYN） by PCR. NES antibody was also used to identify cell immunofluorescence. Result The result of the tree-shrew BM-MSCs differentiation to neuron-like cells with IMEM cell culture differentiation mediums （10 μg/mL EGF and 20 μg/mL bFGF） were good on 12d in-vitro. Conclusion Optimized culture medium with double grow factors and differentiation method were more appropriated for tree shrew BM-MSCs differentiation to neuron-like cells, and the cells differentiated by this manner were consistent to neuron-like cells characteristics.

关 键 词：树鼩 间充质干细胞(MSCs) 体外诱导培养 神经元样细胞 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]