增强化学发光酶联免疫分析用于伏马菌素B_1的快速检测  

Enhanced Chemiluminescence Enzyme-linked Immunoassay for Rapid Detection of Fumonisin B_1

作　　者：刘贝贝 于斐[1] 玉崧成[1] 刘利娥[1] 吴拥军[1,2] LIU Beibei;YU Fei;YU Songcheng;LIU Li＇e;WU Yongjun(College of Public Health, Zhengzhou University, Zhengzhou 450001 , China;College of Chemistry and Molecular Engineering,Zhengzhou University, Zhengzhou 450001 , China)

机构地区：[1]郑州大学公共卫生学院,河南郑州450001 [2]郑州大学化学与分子工程学院,河南郑州450001

出　　处：《郑州大学学报（理学版）》2017年第4期76-81,共6页Journal of Zhengzhou University:Natural Science Edition

基　　金：国家自然科学青年基金项目(81402721)

摘　　要：以抗原-抗体的免疫反应及化学发光为基础,以辣根过氧化物酶(HRP)标记伏马菌素B_1(FB_1)为半抗原、鲁米诺-H_2O_2为发光底物,对羟基联苯为化学发光增强剂,建立了一种新型的FB_1快速检测方法——增强化学发光酶联免疫分析法,相关系数R为0.991 6,线性范围为200~1 400μg/L,检测限为91.3μg/L.在不同的加标水平下,回收率为85.9%~115.4%,与之相对应的板内RSD为9.1%~10.2%(n=6),板间RSD为10.6%~12.4%(n=6),与其他真菌毒素交叉反应率较低.与传统的检测方法(酶联免疫吸附测定和高效液相色谱法)进行了比较,结果表明,本研究建立的方法灵敏度高、特异性好、操作简单、检测快速,能够用于大批量实际样品的快速检测.Based on the immune reaction between antigen and antibody and chemiluminescence, a new method for rapid detection of fumonisin BjWas established using luminol-H202 as luminescent substrates and the hydroxy diphenyl as chemiluminescence enhancer, and named as enhanced chemiluminescence enzyme-linked immunoassay （ ECLEIA）. On the basis of optimizing the experimental conditions, the limit of detection was 91. 3 jxg/L, and the linear range of the ECLEIA method for FBj determination was from 200 jxg/L to 1 400 ｜xg/L, while the correlation coefficient was 0. 991 6. Furthermore, the relative stand-ard deviations of intra-assay and inter-assay were 9. 1 % ？10. 2% （ = 6 ） and 10. 6% ？12. 4% （n =6） , respectively. The recoveries were 85. 9 % ？115. 4% , and the cross-reactivity rates with object ana-logues were low. Compared with other common detection methods （ HPLC and ELISA） , ECLEIA method had high sensitivity, high specificity, sample operation and rapid detection, which could be used for the fast testing of large quantities of real samples.

关 键 词：增强化学发光酶联免疫分析 伏马菌素B1 快速检测 

分 类 号：O657.39[理学—分析化学]