硒矿区土壤中真菌的分离培养及抗肿瘤活性筛选  

Isolation of Fungal Strains in Soil of Selenium Mining Area and Screening for Their Anti-tumor Activity

作　　者：冉喜玲 刘雨 田从魁 朱婷婷 宋雅楠 莫月贤 RAN Xiling;LIU Yu;TIAN Congkui;ZHU Tingting;SONG Yanan;MO Yuexian(Key Laboratory of Biological Resources Protection and Utilization of Hubei Province ＆ Wuling Mountain Institute of Natural Medicine, Hubei University for Nationalities, Enshi 445000;State Environmental Protection Key Laboratory of Drinking Water Source Management and Technology ＆ Shenzhen Academy of Environmental Sciences, Shenzhen 518001)

机构地区：[1]生物资源保护与利用湖北省重点实验室湖北民族学院武陵山天然药物研究所,恩施445000 [2]国家环境保护饮用水水源地管理技术重点实验室深圳市环境科学研究院,深圳518001

出　　处：《北京大学学报（自然科学版）》2017年第6期1161-1164,共4页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：湖北省高校优势特色学科群建设计划(鄂学位[2015]5);生物资源保护与利用湖北省重点实验室重点项目(PKLHB1504);深圳市科技研发资金(JCYJ20130402094730839);湖北民族学院博士启动基金(4138004)和湖北民族学院大学生创新创业项目(2016CX0715)资助

摘　　要：利用单菌落挑选与划线培养技术,从硒矿区土壤样品中分离纯化真菌。通过摇床发酵培养,提取制备发酵样品。采用MTT法,用MDA-MB-231细胞、Hela细胞、SMMC-7721细胞测试发酵样品的抗肿瘤活性。从采自湖北恩施全球唯一独立硒矿床区的6个不同土样中分离真菌73株。其中,对人宫颈癌Hela细胞的IC_(50)小于90μg/m L的菌株32株,对人乳腺癌MDA-MB-231细胞的IC_(50)小于90μg/m L的菌株13株,对人肝癌SMMC-7721细胞的IC_(50)小于90μg/m L的菌株9株。这些活性菌株为筛选抗肿瘤天然产物提供了良好的药源菌株。The fungal strains were obtained by single colony isolation technique from the soil of the world＇s unique proved independent selenium deposit. The anti-tumor activity was assayed by MTT method using MDAMB-231 cells, Hela cells and SMMC-7721 cells, respectively, together with morphological observation of these cells under inversed light microscope. The 73 terrestrial fungal strains were isolated from the soil samples collected from 6 different areas in Enshi area of Hu Bei Province, China. Among them, 32 strains produced fermentation products obviously inhibiting the Hela cells, 13 strains inhibiting the MDA-MB-231 cells and 9 strains inhibiting the SMMC-7721 cells with IC50 value less than 90 μg/ml. These antitumor-metabolite-producing strains have provided fungal strains for further studies on the bioactive metabolites.

关 键 词：真菌 硒矿床区 陆生真菌 分离培养 抗肿瘤活性 

分 类 号：R915[医药卫生—微生物与生化药学]