全封闭一次性小培养基的研制及其在丝状真菌小培养中的应用  

Development of totally enclosed slide culture medium and its application in filamentous fungi culture

作　　者：陈东科[1] 邓存兴 许宏涛[1] 

机构地区：[1]北京医院检验科,北京100730 [2]珠海美华医疗科技有限公司,广东珠海519000

出　　处：《临床检验杂志》2017年第10期739-743,共5页Chinese Journal of Clinical Laboratory Science

摘　　要：目的研制一种安全实用的一次性真菌小培养基。方法参照钢圈小培养法原理。底板选用透明度高的PVC为原材料,同常规使用的载玻片大小(25.2 mm×75.9 mm×1.05 mm),板面两端磨砂设计;圆形培养皿直径19.4 mm,侧面高3.75mm,其上接种孔直径为2.4 mm,可随用户需求灌注PDA琼脂等培养基,加孔塞封闭;上封盖片采用钢化玻璃制成。一次性真菌小培养基用培养湿盒进行保存,两端水槽内加海绵条,加盖密封盖。分别观察临床丝状真菌在该全封闭一次性小培养基中的生长状况、镜下形态及染色效果以及培养基的保存效果。结果该全封闭一次性小培养基实际应用效果良好,真菌生长状况可视、色素明显,镜下可观察到真菌原生态生长形态,可直接进行染料灌注染色,且效果良好。培养基包装后于4℃保存3个月后外观不变,量未见减少。结论成功研制一种操作简单、透明度高、密封效果好、培养性能可靠、生物安全性高的全封闭一次性小培养基。可随时在镜下观察生长状况、适用于油镜下直接观察、可直接进行棉蓝染色,临床试用效果良好,适用于各级实验室的真菌形态学检查。Objective To develop a safe,practical,disposable slide culture medium for fungi culture. Methods Based on the principle of traditional slide culture medium with small steel ring,a plate of polyvinyl chloride（ PVC） with high transparency was chosen as the bottom material of the new medium which held the size of the conventional slide（ 25. 2 mm × 75. 9 mm × 1. 05 mm） with frosted design at both ends. The diameter of inoculation hole was 2. 4 mm on the rounded culture dish which was designed with diameter of 19. 4 mm and side height of 3. 75 mm. The users could inject potato dextrose agar（ PDA） or other medium into the culture dish as needed and then sealed it with a plug. The upper cover was prepared with toughened glass. The improved disposable slide culture medium should be kept in humidifying box with sponge strips in the water tanks of both sides and sealed with cap. The growth status,microscopic morphology and staining result of filamentous fungi in the totally enclosed slide culture medium were observed and the preservative status of the prepared medium was also monitored simultaneously. Results The effects of the improved slide culture medium were satisfactory in clinical practical application. The growing status of fungi could be observed visually and the pigment was clear. The original growth form of fungi could be monitored under microscope and dye material could be perfused directly to stain with good results. The appearance and the volume of packaged slide medium were unchanged after preservation at 4 ℃ for 3 months. Conclusion An improved slide culture medium was successfully developed,which should be easy to operate,high visible,satisfactory for sealing effects and reliable for culture performance with high biological safety. The growth status of fungi could be observed under microscope at any time,and the medium could also be monitored under oil immersion lens directly and stained with cotton blue. The improved medium could be used in morphological examination for fungi in different l

关 键 词：真菌小培养基 全封闭 一次性使用 

分 类 号：R446.5[医药卫生—诊断学]