致犊牛腹泻肠道原虫的分子生物学鉴定  

Identification of Intestinal Protozoa Causing Diarrhea in Calves by Molecular Biology

作　　者：于正青 胡瑞思 王莎莎 赵光辉[1] 宋军科[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《动物医学进展》2017年第11期48-52,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31572509);陕西省科学技术研究发展规划资助项目(2016NY-113);中国博士后面上项目(2016M592848);陕西省博士后科研项目资助特别资助

摘　　要：为确定导致犊牛腹泻的寄生原虫种类及分子学特性,利用基于隐孢子虫核糖体小亚基RNA、蓝氏贾第虫β-贾第素和毕氏肠微孢子虫内转录间隔区的分子生物学技术对腹泻犊牛的粪便样品进行鉴定。结果表明,引起犊牛腹泻的寄生原虫包括微小隐孢子虫、安氏隐孢子虫、瑞氏隐孢子虫、E型蓝氏贾第虫和多种基因型的毕氏肠微孢子虫,并且存在多种原虫混合感染。说明原虫是引起犊牛腹泻的重要病原,畜牧业临床中应加强原虫病的防控。To determine the protozoan species that cause calf diarrhea, molecular techniques based on the small sub-unit ribosomal RNA of Cryptosporidium, β-giardin of Giardia lamblia ,and internal transcribed spacer of Enterocytozoon bieneusi were used to identify the fecal samples of diarrheal calves.The protozoa causing diarrhea of calves included C.parvum, C.andersoni, C.ryanae, type E G.lamblia, and several genotypes of E.bieneusi ,and mixed infection of several protozoa were also observed.The protozoa were the important pathogens causing diarrhea of calves,and controlling the protozoan diseases should be strengthened in the clinic of animal husbandry.

关 键 词：犊牛 腹泻 原虫 鉴定 

分 类 号：S852.72[农业科学—基础兽医学]