猫疱疹病毒Ⅰ型PCR检测方法的建立与初步应用  

Establishment and Preliminary Application of PCR for Detection of Felid Herpesvirus

作　　者：李航[1] 刘俊平[1] 关平原[1] 雷宇[1] 李志 

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]内蒙古呼和浩特市动物卫生监督所,内蒙古呼和浩特010020

出　　处：《动物医学进展》2017年第11期86-90,共5页Progress In Veterinary Medicine

摘　　要：建立猫疱疹病毒Ⅰ型(FHV-1)PCR检测方法,应用于临床样品中FHV-1的快速检测。根据猫疱疹病毒Ⅰ型的gI基因序列设计了一对特异性引物,以疑似猫疱疹病毒Ⅰ型感染猫的眼鼻分泌物总DNA为模板,建立了PCR检测方法,对所建立的方法进行特异性、敏感性和重复性验证,并对26份临床样本进行检测。结果表明,所建立的PCR诊断方法与犬细小病毒(CPV)、犬瘟热病毒(CDV)、绵羊肺炎支原体(M.ovis)、牛支原体(M.bovis)、牛疱疹病毒Ⅰ型(BHV-1)均无交叉性反应,最低检测DNA模板浓度为3.23×10~3 copies/μL。对26份临床病例进行检测,阳性率61.54%。说明建立的FHV-1PCR检测方法具有特异性强、敏感度高、方便快捷等优点,适合于临床FHV-1感染的快速检测。This study aimed to establish a PCR diagnostic method to feline herpesvirus-1 （FHV-1）,which provided a quick test to the clinical specimen.This study designed a pair of specific primers based on the gl genetic sequence of FHV-1,the template used in PCR was the total DNA extracted from occulonasal discharge of cats with suspicious FHV-1 infection. Then,we carried out test to 26 clinical specimen for the specificity, sensitivity,and repeatability.The result showed that the PCR test had no cross reaction with canine parvovirus,cainine distemper virus,Mycoplasma ovis,Mycoplasma boris.The lowest DNA template test concentration was 3.23× 10^2 copies/μL.The positive rate was 61.54%.As a result,this test had high specificity and sensitivity,and can be used to detect clinical infections with FHV-1.

关 键 词：猫 猫疱疹病毒Ⅰ型 PCR检测 

分 类 号：S852.659.1[农业科学—基础兽医学]