苦参碱和甘草酸的小肠吸收特性分析  

Analysis of Intestinal Absorption Characteristics of Matrine and Glycyrrhizic Acid

作　　者：闫涛[1] 姜维[1] 王琴会[1] 穆薇[1] 石磊[1] 

机构地区：[1]第四军医大学唐都医院药剂科,西安710038

出　　处：《中国药师》2017年第11期1906-1910,共5页China Pharmacist

基　　金：国家自然科学基金项目(编号:81503327)

摘　　要：目的:研究苦参碱和甘草酸在小肠各段和不同剂量下的吸收特征,探索两种成分联用时的吸收变化。方法:采用大鼠单向肠灌流模型,利用HPLC法测定灌流液中苦参碱和甘草酸的含量变化,计算其吸收速率参数(K_a)和有效渗透系数(P_(eff))。结果:苦参碱在小肠各段均有吸收且空肠段吸收最好与十二脂肠和回肠的吸收参数差异有统计学意义(P<0.05);不同质量浓度下吸收差异有统计学意义(P<0.05)。甘草酸在小肠吸收较差,不同肠段和质量浓度下的吸收差异无统计学意义(P>0.05)。两种物质联用时,苦参碱和甘草酸的吸收与单用时相比差异有统计学意义(P<0.05)。结论:苦参碱的吸收呈剂量相关性,随质量浓度的增加而增加。甘草酸的吸收不随质量浓度变化而改变,吸收方式可能为被动转运。两种物质联用时可以提高苦参碱的体内吸收和甘草酸的肠内浓度。Objective： To investigate the absorption characteristics of matrine and glycyrrhizic acid in different intestinal segment and at various mass concentration, and explore the absorption changes of the combination of the two drugs. Methods： In situ single pass intestinal perfusion （SPIP） model was used, and the concentration changes of matrine and glycyrrhizic acid were determined by HPLC, and Ka and Peff were calculated. Results： Matrine was absorbed in the whole of small intestine, and best in jejunum, and compared with the duodenum and ileum, the absorption had significant difference （ P 〈 0.05 ） . The absorption at different mass concentrations had significant difference（P 〈0.05）. The absorption of glycyrrhizic acid in small intestine was poor without significant differences in different intestinal segments and at various doses （P 〉 0.05）. After the combination of the two drugs, the absorption of matrine and glycyrrhizic acid had significant difference when compared with that of separated use （P 〈 0.05）. Conclusion： The absorption of matrine is dose-dependent, and with the dose increase, the absorption increases. Glycyrrhizic acid absorption does not change with mass concentration, and the absorption may be passive transport. The combination of the two drugs can improve the in vivo absorption of matrine and increase the concentration of glycyrrhizic acid in intestine.

关 键 词：苦参碱 甘草酸 在体单向肠灌流 高效液相色谱法 肠吸收 

分 类 号：R945[医药卫生—微生物与生化药学]