伏立康唑磺丁基醚-β-环糊精包合物滴眼液治疗兔真菌性角膜炎的实验研究  

Effects of Voriconazole Sulfonated Butyl Ether-β-Cyclodextrin Inclusion Compound Eye Drop on Fungal Keratitis in Rabbits

作　　者：胡拥军[1] 宋玲[1] 张霁霁 

机构地区：[1]湖北省妇幼保健院药剂科,武汉430070 [2]荆州市第一人民医院

出　　处：《中国药师》2017年第11期1942-1944,共3页China Pharmacist

摘　　要：目的:对伏立康唑磺丁基醚-β-环糊精包合物(VCZ-SBE)滴眼液对兔真菌性角膜炎的治疗作用进行考察。方法:采用烟曲霉菌建造家兔真菌性角膜炎(FK)模型。48只健康家兔,随机分为4组。A组12只右眼接种烟曲霉菌,不做任何治疗;B组12只右眼接种烟曲霉菌成功后当天开始滴用0.5%VCZ-SBE滴眼液;C组12只右眼接种烟曲霉菌成功后当天开始滴用1.0%VCZ-SBE滴眼液;D组12只右眼接种烟曲霉菌成功后当天开始滴用0.5%氟康唑滴眼液。随机取以上各组家兔健康左眼角膜作为空白对照。各组建模成功后当天及第1,4,7,14天进行溃疡评分,并于第1,4,7,14天各组处死3只家兔,取其角膜,观察炎症细胞浸润程度及真菌菌丝侵入深度。结果:各组兔右眼烟曲霉菌接种成功后溃疡评分差异无统计学意义(P>0.05)。B组于治疗后的第7天溃疡评分明显低于A组(P<0.05),C组于治疗后的第4天溃疡评分明显低于A组(P<0.05),D组于治疗后的第14天溃疡评分明显低于A组(P<0.05)。在治疗后的第4、7、14天,C组的溃疡评分、炎症浸润深度及坏死程度均明显低于B、D组(P<0.05),而B组在治疗后的第7,14天的以上指标明显低于D组(P<0.05)。结论:与同浓度的氟康唑滴眼液相比,VCZ-SBE包合物滴眼液可更好地降低真菌性角膜炎的溃疡评分,降低浸润深度,且随着浓度的增加,药效增强。Objective： To study the effects of voriconazole sulfonated butyl ether- [^-cyclodextrin inclusion compound （VCZ-SBE） eye drop on fnngal keratitis （FK） in rabbits. Methods： Aspergillus fumigatus was used for the establishment of FK model in rabbits. Totally 48 healthy rabbits were divided into four groups. The 12 right eyes in group A were inoculated with Aspergillus fumigatus without any treatment. The 12 right eyes in group B were inoculated with Aspergillus fumigatus, and then treated with 0.5% VCZ-SBE eye drop, those in group C were inoculated with Aspergillus fumigatus, and then treated with 1.0% VCZ-SBE eye drop, and those in group D were inoculated with Aspergillus fumigatus, and then treated with 0.5% fluconazole eye drop. The cornea of normal left eyes in the four groups was used for the blank control. Ulcer grading was performed for all the eyes in 0, 1,4, 7 and 14 d after the successful establishment of FK model. Every 3 rabbits were sacrificed in 1,4, 7 and 14 d, and then the cornea was obtained to evaluate the infiltration degree of inflammatory cells and the invasion depth of hyphae into cornea. Results： After the successful establishment of FK model, the ulcer grading among the groups showed no significant difference （P 〉 0.05）. Respectively after the 7, 4 and 14-day treatment, the ulcer grading in group B, C and D was notably lower than that in group A （P 〈 0.05）. After the 4, 7 and 14-day treatment, the ulcer grading, infiltration depth and necrosis degree in group C were all lower than those in group B and D （P 〈 0.05）, and after the 7 and 14-day treatment, those in group B were lower than those in group D （P 〈 0.05）. Conclusion： Compared with fluconazole eye drop at the same concentration, VCZ-SBE inclusion compound eye drop can decrease the ulcer grading of FK and reduce the infiltration depth in a concentration-dependent manner.

关 键 词：伏立康唑 磺丁基醚-Β-环糊精 包合物 滴眼液 真菌性角膜炎 氟康唑 

分 类 号：R965.1[医药卫生—药理学]