健脑益寿胶囊对阿尔茨海默病小鼠学习记忆和海马毛细血管结构的影响  

Effects of Jiannao Yishou Capsule on Learning and Capillary Structure of Hippocampal in Alzheimer's Disease Mice

作　　者：苏慧[1] 费洪新[2] 张英博[2] 苏云明 

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040 [2]齐齐哈尔医学院基础部

出　　处：《中国药师》2017年第11期1972-1975,共4页China Pharmacist

基　　金：黑龙江青年科学基金项目(编号:QC2014C119);黑龙江省齐齐哈尔市科技局项目(编号:SFGG-201630);黑龙江齐齐哈尔医学院院内基金项目(编号:QY2016B-26)

摘　　要：目的:研究健脑益寿胶囊(JYC)对阿尔茨海默病(AD)模型小鼠海马的保护作用。方法:雄性小鼠随机分成正常对照组、模型对照组、阳性对照组(盐酸多奈哌齐片,0.001 g·kg^(-1))、JYC高(3.744 g·kg^(-1))、低(0.936 g·kg^(-1))剂量组,采用Morris水迷宫用于测试小鼠学习和记忆能力,采用光镜和电镜观察海马毛细血管结构,使用酶联免疫法测定海马Aβ水平。结果:与正常对照组比较,模型对照组小鼠学习记忆明显降低,差异有统计学意义(P<0.05),毛细血管内皮细胞线粒体变性、核固缩、核周间隙增宽、重叠连接不清晰,海马Aβ水平明显升高,差异有统计学意义(P<0.05);与模型对照组比较,JYC高剂量组小鼠学习记忆明显升高,差异有统计学意义(P<0.05),海马毛细血管内皮细胞形态明显改善,海马Aβ水平明显降低,差异有统计学意义(P<0.05)。结论:JYC能维持AD小鼠海马毛细血管正常形态结构,降低海马Aβ水平并改善学习记忆,对AD小鼠海马具有保护作用。Objective： To investigate the protective effects of Jiannao Yishou capsule （JYC） on hippocampal in Alzheimer＇s disease （AD） mice. Methods： Male mice were randomly divided into normal control group, model control group, positive control group （donepezil hydrochloride tablets, 0. 001 g·kg-1 ） ,JYC high-dose （3. 744 g· kg-1） and low-dose （0. 936 g·kg-1 ） group. Morris water maze was used to test the learning and memory ability of mice. The hippocampal capillary structure was observed by light microscopy and electron microscopy and the level of hippocampal Aβ was measured by enzyme-linked immunoassay. Results ： Compared with the normal control group, the model control group learning and memory significantly reduced, and capillary endothelial cell mitochondrial degeneration, nuclear condensation, broadening of the perinuclear space, overlapping, Aβ in hippocampal of AD mice were sig- nificantly reduced （P 〈 0.05 ）. Compared with the model control group, the JYC high-dose group learning and memory of mice improved significantly （ P 〈 0.05 ） , and hippocampal capillary endothelial cell morphology improved significantly, and Aβ in Hippocampal of AD mice were significantly reduced （ P 〈 0.05 ）. Conclusion ： JYC can maintain normal morphological structure of hippocampus capillaries of AD mice, decrease Aβ responses and Improve learning and memory. JYC has protective effects on hippocampal tissues in AD mice.

关 键 词：健脑益寿胶囊 阿尔茨海默病 学习记忆 

分 类 号：R285.5[医药卫生—中药学]