土壤中4种杀虫剂的高效液相色谱分析  

HPLC analysis of four insecticides in soil

作　　者：黄超福 何宁祖 黄思媚 贾豹 潘加承 

机构地区：[1]南宁市白蚁防治所,广西南宁530023

出　　处：《中华卫生杀虫药械》2017年第5期419-421,共3页Chinese Journal of Hygienic Insecticides and Equipments

基　　金：南宁市科技攻关计划项目(编号:20153128)

摘　　要：目的建立土壤中联苯菊酯、氯菊酯、毒死蜱、吡虫啉的高效液相色谱分析方法。方法使用ODSC18色谱柱和可变紫外光检测器,以甲醇-乙腈-水(体积比70∶21∶9)为流动相,流速1.0 ml/min,在220 nm波长下,对土壤中联苯菊酯、氯菊酯、毒死蜱、吡虫啉进行定量分析。结果 4种农药在5~100 mg/L范围内线性相关系数≥0.999 7,检出限为0.04~0.60 mg/L;添加水平在5~50 mg/kg范围内,平均回收率为89%~102%,相对标准偏差为2.1%~4.1%。结论该方法具有快速、简便、精确度和准确度高、线性关系好的优点,是较理想的分析方法。Objective To establish high-performance liquid chromatography（HPLC） method for the determination of permethrin,cypermethrin,chlorpyrifos and imidacloprid. Methods Quantitative analysis of the active ingredient was conducted using a ODS-C18 chromatographic column,a variable wavelength UV detector at UV 220 nm,methanol-acetonitrile-water = 70 ∶ 21 ∶ 9（by vol） as the mobile phase with the flow rate of 1. 0 ml/min. Results The developed method was linear over the range assayed（5-50 mg/L）,with determination coefficients≥0. 999 7 and the limits of detection between 0. 04 and 0. 60 mg/L. The recoveries for all the pesticides studied were from 89% to 102%with relative standard deviation（RSD） from 2. 1% to 4. 1% in the concentration range of 5-50 mg/kg.Conclusion This method was an ideal method of analysis with the advantages of fast,simple,high accuracy,high accuracy and good linearity.

关 键 词：联苯菊酯:氯菊酯 毒死蜱 吡虫啉:高效液相色谱 定量分析 

分 类 号：S482.3[农业科学—农药学]