蓝莓花青素对由H_2O_2诱导人脐静脉内皮细胞氧化应激损伤的保护作用  

Protective Effect of Blueberry Anthocyanins on Oxidative Stress Injury by H_2O_2 of Human Umbilical Vein Endothelial Cells

作　　者：赵凯[1] 郑源强[2] 贾宇臣[2] 

机构地区：[1]呼和浩特市第一医院,010030 [2]内蒙古医科大学分子生物学研究中心,呼和浩特010059

出　　处：《医学研究杂志》2017年第10期113-115,共3页Journal of Medical Research

基　　金：内蒙古自治区科技厅科技计划项目(20120402);内蒙古自治区研究生科研创新基金资助项目(B20161012615)

摘　　要：目的通过建立人脐静脉内皮细胞株(human umbilical vein endothelial cells,HUVECs)氧化应激损伤细胞模型,探讨蓝莓花青素是否能够对H_2O_2引起的细胞损伤具有保护作用。方法采用过氧化氢(H_2O_2)创建ECV304氧化应激损伤细胞模型,实验分为正常对照组、H_2O_2组、蓝莓花青素低、中、高剂药物组,药物组中加入蓝莓花青素培养2h后,再加入H_2O_2继续培养6h,采用MTT法检测细胞活力,流式细胞术检测细胞周期变化情况及细胞凋亡率。结果细胞活力与H_2O_2浓度及其作用的时间呈相关性下降趋势,在一定浓度范围内蓝莓花青素对细胞具有保护作用。结论蓝莓花青素对由H_2O_2引起的氧化应激损伤具有一定保护作用。Objective To study the protective effect of blueberry anthocyanins on H202 induced human umbilical vein endothelial cells,HUVECs. Methods The cultured cells were divided into 3 groups： normal control group, H202 model group, blueberry anthocya- nins group. MTT method was used to observe cell viability. Annexin V -FITC/PI straining was used to observe cell apoptosis. Flow cy- tometry was used to detect cell cycle. Results H20~could induce HUVECs oxidative stress injury. Cell survival rate was positive correla- ted with drug concentration and action time. In the certain concentration range blueberry anthocyanin had a protective effect. Conclusion Blueberry anthocyanin can protect H202induced oxidative stress injury in HUVECs.

关 键 词：蓝莓花青素 氧化应激损伤 人脐静脉内皮细胞株 

分 类 号：R543[医药卫生—心血管疾病]